Key Words:Uroporphyrin isomer separation HPLC uroporphyrin free acid Uroporphyrin isomers in urine Summary A synthetic mixture of uroporphyrin isomers I, 11,111 and IV as free acid sin the syntheticratio of 1 :1:4:2 was resolved byreversephase HPLC using a C0:PEL (ODS) 37-50 micron precolumn and a Micro Bondapak C18 analytical column eluted with acetonitrile (4%) in phosphate buffer (pH 6.95). Clinically important Iand 111 isomers of uroporphyrin were readily resolved directly from acidified urine as porphyrin free acids.

Avian myeloblastosis virus (AMV) genomic RNA was translated in vitro to identify the AMV transforming gene (myb) product(s). Full-length RNA yielded, in addition to the expected Pr76^gag, a gag-related product of 92,000 daltons which did not appear to be a gag-oncogene fusion protein. Subgenomic AMV RNA in vitro translation, nonstructural products (specific for AMV) were of 54,000, 49,000 and 34,000 daltons. Based on their sizes and similar peptide maps for the smaller two proteins, it is proposed that they derive from the myb region of AMV.

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