Extra-virgin olive oil (383 samples; EVOOs) of three consecutive harvesting years from nine Italian regions were collected and submitted to an 1H NMR-chemometric protocol to characterize the samples according to their origin (geographical area and variety). A more complete assignment of the olive oil 1H spectrum in CDCl3 and DMSOd6 was reported identifying 24-methylencycolartanol. A single classification model provided the discrimination of EVOOs among the three geographical macro-areas (North, Islands, Center-South), whereas a hierarchical approach based on breaking the overall classification problem into a series of smaller linear discriminant analysis (LDA) sub-models was tested to differentiate olive oils according to their geographical regions. Specific compounds responsible for olive oil characterization were identified.

High field NMR characterization of carboxymethyl scleroglucan (Scl-CM) recently synthesized through reaction of the native polymer (Scl) with chloroacetic acid in basic conditions is discussed, reporting the position of the carboxymethyl groups in the scleroglucan moiety. The comparison between the Scl-CM and the Scl structures can be useful to comprehend their different profiles in the viscosity-shear rate curves and their different ability to form physical gels