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The high numbers of primary producers represent multiple sources of organic matter accumulating onto lake bottoms. The difficulty of distinguishing the relative contribution to the mixture presents considerable challenges to the analysis of these organic deposits. In this study, dual-stable isotope analysis and IsoSource model were used to identify allochthonous and autochthonous components of detritus deposits (Particulate Organic Matter: POM) at two different bottom slope sites of a volcanic lake (lake Bracciano). Experiments were carried out to calibrate IsoSource on constructed plant mixtures and assess changes in isotope ratios during plant decomposition. IsoSource satisfactorily discriminated the constructed mixture sources with a few exceptions. Changes in isotopic enrichment during decomposition were low, and thus did not represent a confounding variable in the isotopic analysis. By contrast, chemical and geological differences of the study sites were associated with differences in plant delta(13)C and delta(15)N values (more than 2aEuro degrees within single plant species). At both sites, the isotopic signals of POM fell between polygons delineated by source end members with an evident shift of delta(13)C toward allochthonous sources. POM amount and diversity were greater at the flatter bottom site, where allochthonous contributions were larger than at the other site. In particular, IsoSource ranked species contributions as follows: A. glutinosa > P. australis > A. donax > S. alba > P. nigra > the benthic macroalga Chara sp. at the first site, and A. glutinosa > P. nigra > the aquatic macrophyte C. demersum at the latter. The composition of littoral POM was determined by allochthonous sources in proportion to their relative abundances (as percent land cover) with differences between sites due to bottom slope

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The movement between alfalfa and maize of three of the most common aphidophagous coccinellids in southern Europe was determined using carbon and nitrogen stable isotope analysis at four periods during the growing season. The isotope analysis revealed movement between alfalfa and maize but patterns were different between the three species. While Coccinella septempunctata L. (Coleoptera: Coccinellidae) only moved from alfalfa to maize, Propylea quatuordecimpunctata L. (Coleoptera: Coccinellidae) continuously dispersed between the two adjacent crops. Hippodamia variegata Goeze (Coleoptera: Coccinellidae) showed an intermediate pattern. Results suggest that, while alfalfa acts as a source of the three species to maize, maize also plays a role as a source of P. quatuordecimpunctata and H. variegata to alfalfa. The specific movement traits that emerged for the three coccinellid species suggest that crop management affects them differently and point out that the association between crops is very valuable for improving coccinellid biological control services.