Environmental pollution is one of the major problems for human health. Toxic heavy metals are normally present as soil constituents or can also be spread out in the environment by human activity and agricultural techniques. Soil contamination by heavy metals as cadmium, highlights two main aspects: on one side they interfere with the life cycle of plants and therefore reduce crop yields, and on the other hand, once adsorbed and accumulated into the plant tissues, they enter the food chain poisoning animals and humans. Considering this point of view, understanding the mechanism by which plants handle heavy metal exposure, in particular cadmium stress, is a primary goal of plant-biotechnology research or plant breeders whose aim is to create plants that are able to recover high amounts of heavy metals, which can be used for phytoremediation, or identify crop varieties that do not accumulate toxic metal in grains or fruits. In this review we focus on the main symptoms of cadmium toxicity both on root apparatus and shoots. We elucidate the mechanisms that plants activate to prevent absorption or to detoxify toxic metal ions, such as synthesis of phytochelatins, metallothioneins and enzymes involved in stress response. Finally we consider new plant-biotechnology applications that can be applied for phytoremediation.Key words: cadmium; heavy metals; metallothioneins; phytochelatins; phytoremediation; transporters. Available online at www.jipb.net As sessile organisms plants have restricted mechanisms for stress avoidance and are subjected to environmental stresses that change growth conditions and alter (or sometimes disrupt) their metabolic homeostasis. Worldwide, these stresses are the most limiting factors for crop productivity: a large proportion of annual yield is lost due to pathogen attack and to unfavorable abiotic conditions such as drought, salinity and extreme temperatures. The average and record yields of many crops were compared in a classical study (Boyer 1982) and it was found that crop plants were reaching only 20% of their genetic yield potential. Diseases, insects and weeds contributed only in part, with the major yield reduction resulting from abiotic stresses. Therefore, understanding how plants cope with stresses and

During their life, plants have to cope with a variety of abiotic stresses. Cadmium is highly toxic to plants, water soluble and therefore promptly adsorbed in tissues and its presence greatly influences the entire plant metabolism. In this review, we focus on the signal pathways responsible for the sensing and transduction of the "metal signal" inside the cell, ultimately driving the activation of transcription factors and consequent expression of genes that enable plants to counteract the heavy metal stress.

An experimental system has been developed which allows the identification of intermediates in the abscisic acid (ABA) signal transduction pathway leading to desiccation tolerance in plants. Desiccation tolerance in callus of the resurrection plant Craterostigma plantagineum is mediated via the plant hormone ABA, which induces the expression of gene products related to desiccation tolerance. Based on T‐DNA activation tagging, a gene (CDT‐1) was isolated which encodes a signalling molecule in the ABA transduction pathway. Constitutive overexpression of CDT‐1 leads to desiccation tolerance in the absence of ABA and to the constitutive expression of characteristic transcripts. CDT‐1 represents a novel gene with unusual features in its primary sequence.The CDT‐1 gene resembles in several features SINE retrotransposons. Mechanisms by which CDT‐1 activates the pathway could be via a regulatory RNA or via a short polypeptide.

Mineral nutrition of plants greatly depends on both environmental conditions, particularly of soils, and the genetic background of the plant itself. Being sessile, plants adopted a range of strategies for sensing and responding to nutrient availability to optimize development and growth, as well as to protect their metabolisms from heavy metal toxicity. Such mechanisms, together with the soil environment, meaning the soil microorganisms and their interaction with plant roots, have been extensively studied with the goal of exploiting them to reclaim polluted lands; this approach, defined phytoremediation, will be the subject of this review. The main aspects and innovations in this field are considered, in particular with respect to the selection of efficient plant genotypes, the application of improved cultural strategies, and the symbiotic interaction with soil microorganisms, to manage heavy metal polluted soils.

Heavy metals are often present naturally in soils, but many human activities (e.g. mining, agriculture, sewage processing, the metal industry and automobiles) increase their prevalence in the environment resulting in concentrations that are toxic to animals and plants. Excess heavy metals affect plant physiology by inducing stress symptoms, but many plants have adapted to avoid the damaging effects of metal toxicity, using strategies such as metal chelation, transport and compartmentalization. Understanding the molecular basis of heavy metal tolerance in plants will facilitate the development of new strategies to create metal-tolerant crops, biofortified foods and plants suitable for the phytoremediation of contaminated sites.