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the use of metal nanoparticles as supplements of animal diets does not always bring unambiguous results. there are many reports in the literature about the multifaceted effects of this type of supplementation on the animal organism. therefore, the aim of the paper is to present the current knowledge of the possible application of nanometal forms in animal nutrition and its potential benefits and threats. The positive effect of nanoparticles used as feed additives has most frequently been reflected in an increase in body weight, higher average daily gain, or improvement of the FCR value. In some cases, however, the effect of nanoparticle addition to diets was indiscernible. The potent antibacterial activity of nanoparticles, especially against Gram-negative bacteria and Gram-positive bacteria, is regarded as a positive effect. In turn, the probability of their toxicity is a potential risk in application thereof. Supplementation of diets with nanometals has been accompanied by pathological changes in animal tissues, primarily in the pancreas, kidney, liver, rumen, abomasum, small intestine, adrenal glands, and brain. additionally, at the the cellular level, nanoparticles were found to induce toxicity, inflammatory excitation, and cell death. Oral administration of nanoparticles induced a risk of malfunction of the nervous system and even impairment of cognitive processes in animals. The increasing knowledge of the possible toxic effects of nanoparticles on the animal organism suggests caution in their use in animal production and necessitates further precise investigations in this area.

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The studies were conducted in a forest settlement in Roztocze National Park (eastern Poland). The aim of the study was to evaluate the vegetation of two pastures depending on the type of use in the context of grassland protection, identify the trends of species composition changes, and analyse the yield and nutritional value of the biomass in the context of animal welfare. The studies were conducted on permanent grasslands varying in terms of fertility and location. Both sites were pastures where native breeds of livestock (Polish Lowland sheep of the Uhrusk and Polish Konik) were grazing. The fertile pasture was represented by the developing Lolio-Cynosuretum association, while the poor dry pasture -by a community with Common Bent (Agrostis capillaris L.) and a community with Mouse-Ear Hawkweed (Hieracium pilosella L.). The greatest changes over time were observed in the mowed site in the fertile pasture (increased share of tall grasses) and in the abandoned poor dry pasture (increased share of herbs and weeds). Livestock grazing conducted from 2010 influenced the stabilisation of the species composition. Tree and shrub seedlings were systematically eaten by livestock, which evidences a positive impact of grazing on the preservation of permanent grasslands in Roztocze National Park where forest ecosystems predominate. The assessment of the species composition and yielding indicated that the fertile pasture was characterised by good value while the poor pasture -low or sufficient value. In terms of nutrient yield and content, these pastures were poor or very poor, and their nutritive potential was largely dependent on the meteorological conditions. While the livestock density in the pastures, ranging from 1.1 to 1.5 LSU ha -1 in the years under study, was appropriate, grazing should be limited in the summer months, particularly in periods of drought, by reducing the number of animals or by additional feeding to ensure their welfare.

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