Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL -1 , 4 mg mL -1 , 6 mg mL -1 , 8 mg mL -1 and 10 mg mL -1 ) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml -1 ) as negative control. Cell viability was calculated as: absorbance average in treatments/ absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml -1 and 10 mg ml -1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori's trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL -1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL -1 extract in the in vivo study. Thus, we conclude that the 2mg mL -1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro. Key words: Wound healing. Rabbits. Picrosirius. Gomori trichome. Wheat. ResumoOs extratos vegetais têm sido utilizados na cicatrização de feridas a muitos séculos. No entanto nos dias atuais a comprovação científica dos fitoterápicos é uma exigência da legislação. Na literatura científica se observa a necessidade de cicatrizantes tópicos que proporcionem uma cicatriz estética e resistente. Devido a isso objetivou-se avaliar a citotoxicidade de diversas doses de T. aestivum (2 mg mL 1 , 4 mg mL -1 , 6 mg mL -1 , 8 mg mL -1 e 10 mg mL -1 ) em linhagem celular de fibroblasto, e o processo cicatricial em modelo experimental (New Zealand rabbits) in vivo. Para isso foi realizado o teste de MTT em linhagem celular 3T. Tests were performed in duplicates, using MEM (0 mg mL -1 ) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average

Leptin acts on energy metabolism, affecting reproductive functions through activation of its receptors in the brain and in reproductive organs. This study compared the presence of leptin and its receptor (ObR-b) in hypothalamus neurons, endometrial glands and oocytes of culled swine females across ovarian statuses and parities. Immunohistochemistry was done in samples of uterus, ovaries and hypothalamus from 28 culled females, using polyclonal antibodies antileptin and ObR-b. Immunolabelling was compared for sows categorized by parity at culling (0, 1, 2-4 and <4) and ovarian status (luteal and follicular phases of the oestrous cycle and with cysts). Immunolabelling for leptin and ObR-b in neurons and oocytes was weaker in females with cysts (p < 0.05) than in those at the follicular phase. In endometrial glands, leptin immunolabelling was less intense in females with cysts (p < 0.05), but immunolabelling for ObR-b was similar across ovarian statuses (p > 0.05). In sows culled with 2-4 parities, leptin immunolabelling in neurons and endometrial glands was more intense than in nulliparous females (p < 0.05). In comparison with sows culled at greater parities, ObR-b immunolabelling for nulliparous females was less intense in endometrial glands and in oocytes (p < 0.05), but more intense in neurons (p < 0.05). Thus, in swine, the presence of leptin and ObR-b varies across parities and is more intense in the uterus, ovaries and hypothalamus of females that were cycling before culling than in those having cystic ovaries.

This paper analyzes the influence of infrared radiation (IR) on regeneration, after autotomy of limb buds of Neohelice granulata and consequently the time molt. Eyestalks were ablated to synchronize the start of molt. Afterward, animals were autotomized of five pereopods and divided into control and irradiated groups. The irradiated group was treated for 30 min daily until molt. Limb buds from five animals of days 4, 16 and 20 were collected and histological sections were made from them. These sections were photographed and chitin and epithelium content measured. Another group was made, and after 15 days limb buds were extracted to analyze mitochondrial enzymatic activity from complex I and II. The irradiated group showed a significant reduction in molt time (19.38+/-1.22 days) compared with the control group (32.69+/-1.57 days) and also a significant increase in mitochondrial complex I (388.9+/-27.94%) and II (175.63+/-7.66%) in the irradiated group when compared with the control group (100+/-17.90; 100+/-7.82, respectively). However, these effects were not accompanied by histological alterations in relation to chitin and epithelium. This way, it was possible to demonstrate that IR increases complex I and II activity, reduces the time molt and consequently increases the appendage regeneration rate.

No abstract

A mortalidade neonatal de cães leva a perdas econômicas e afetivas. Na primeira semana de vida, os filhotes são mais suscetíveis a infecções porque não possuem sistema imunológico e microflora satisfatórios. A identificação da etiologia da morte fetal e neonatal depende do exame histopatológico e identificação de agentes infecciosos. O objetivo deste estudo foi relatar um caso de septicemia causada por Streptococcus canis em um neonato canino de quatro dias, enviado para exame de necropsia. O veterinário responsável relatou que o canino apresentava apatia, vocalizações e aumento do volume abdominal. Na necropsia, o canino apresentava persistência da veia umbilical, extensas áreas esbranquiçadas e friáveis no fígado, aumento do volume do estômago, contendo grande quantidade de gás no seu interior, e intestinos com presença de gases e avermelhamento do hemisfério cerebral direito. O exame histopatológico demonstrou extensas áreas multifocais de necrose hepática com inúmeras bactérias, por vezes presentes no interior de vasos sanguíneos (embolia bacteriana), além de encefalite discreta. Em amostras de tecido para cultura bacteriana, a bactéria S. canis foi isolada. O cuidado neonatal precoce é essencial para reduzir as perdas e combater as principais vulnerabilidades do neonato canino, que consiste em baixa termorregulação, risco de desidratação, hipoglicemia e imaturidade imunológica. Em vista de uma abordagem terapêutica correta para reduzir a mortalidade neonatal, o exame post mortem se torna uma ferramenta importante para identificar os possíveis agentes causadores, sejam eles virais, bacterianos ou outras patologias que vão além da tríade neonatal. O S. canis está associado a infecções esporádicas e oportunistas em cães.