Liver is one of the largest gland of the body and the main site for intense metabolism and  excretion. Liver is concerned with metabolism of endogenous as well as exogenous substances. “Withania somnifera” and “Rubia cordifolia” claim to have hepatoprotective action. Till now, no scientific study has been performed to evaluate such claims. This was an experiment study conducted on wistar rats, in Department of Pharmacology, Index Medical College.  Healthy Albino wistar rats  of either gender, weighing 150- 200g were obtained from CPCSEA approved Central Animal House. The selected rats were housed in polpropylene cages under controlled conditions of temperature (25 0C) and alternating periods of light and darkness of 12 hours each. The rats had free access to standard rat pellet diet and tap water ad libitum. After one week of acclimatization, the animals were rendered suitable for study. Pregnant female rats were not included in the study. ALT level in normal saline treated group was 29.5±3.35 IU/L. It was found to be significantly increased (p<0.001) with administration of CCl4 to 433.5±48.67 IU/L.

Liver is one of the largest glands of the body and the main site for intense metabolism and excretion. Liver disease is a term for a collection of conditions that adversely affect the cells, tissues, structures, or functions of the liver.  Treatment of liver diseses with various drugs develops risk of toxicity. Rubiacordifolia is an herbal plant has, been claimed to have hepatoprotective potentials. The present study was conducted to explore the hepatoprotective activity of aqueous extract of Rubiacordifolia against carbon tetrachloride induced hepatotoxicity in albino rats. This experimental study was conducted after getting approval from institutional animal ethics committee using, routes of Rubiacordifolia in wistar albino rats (150-200g) of either sex. The hepatoprotective activity was evaluated by using histo-pathological examination. Animals were divided in to five groups with six animals in each group. Group-1 was given Normal saline (1ml/kg/day), Group-2 was injected with carbon tetrachloride (1ml/kg) i.p. only once to produce hepatotoxicity. Group-3 and Group-4 were given    Rubiacordifolia (100mg/kg and 200mg/kg) orally every morning for 21 days followed by carbon tetrachloride (1ml/kg) i.p on 21st day (respectively).