Background: Hepatic disorders though prevalent in cattle especially in calves received negligible attention for their definitive diagnosis. Clinical evaluation of liver remained dependent on laboratory estimation of hepato specific biochemicals which often lead to misdiagnosis. The present detailed three phase study was aimed at determining ultrasonographic evaluation of liver and its associated structures in calves up to 9 months of age to develop a baseline data that could be used for their diagnostic evaluation.Methods: Calves were divided in three groups viz. neonatal, preruminant and ruminating; Cadaveric and in-vitro organ bath ultrasonographic studies (n=2 in each group) was followed by comparative real time in-vivo ultrasonography on live calves (n=6 in each corresponding group).Result: Liver was seen in right 5th to 11th Intercostal space (ICS) in most neonatal calves, 6th to 11th ICS in preruminant calves and from 8th ICS to the area just beyond the last rib in ruminating calves. Parenchyma appeared smooth isoechogenic intercepted with portal vein branches. Echobiometric parameters showed statistically significant difference among three groups of animals. Gallbladder appeared pyriform depicted mostly in 9th ICS. Caudal vena cava appeared triangular and was seen at 9th to 11th ICS, except in ruminating calves. Portal vein was prominent in 10 ICS. Amongst identical age groups, the comparative parameters, resembled largely in different studies.