Sea cucumber (Holothuria atra) is one of the bioresources that has not been utilized optimally. This marine animal contains potentially bioactive compounds, therefore it can be used as an alternative by fishery products processing industries. This study was aimed to determine the potential of the bioactive compound in sea cucumber and to determine the antibacterial activity of sea cucumber against Pseudomonas aeruginosa. Experiments were carried out using diffusion method with concentration 0.50, 0.75, 1 mg/disc. The results showed that methanol extract of sea cucumber contained terpenoids, saponins, and phenolic. The diameter of methanol extract inhibition zone at 1 mg concentration of 12.25±0.05 mm and of three types of fractions used hexane fraction had a more dominant antibacterial potency with diameter inhibition zone at 1 mg concentration of 14.61±0.02 mm. Based on the results of research that the methanol extract and hexane fraction of sea cucumber has potential as an antibacterial.

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