Problem of fruit oblongation arises in nagpur mandarin fruits from last decades and it is dominantly increasing from last 4 to 5 years in nagpur mandarin growing regions of Akot, Amravati, Wardha, Nagpur and some part of Madhya Pradesh. The present investigation was carried out at Post Harvest Technology Laboratory, Department of Horticulture, Dr PDKV, Akola in order to study the physicochemical characteristics of physiologically disordered nagpur mandarin fruits. Samples of physiologically disordered fruits and normal fruits were collected from twenty-five orchards of five different locations (viz., Ruikhed, Anjangaon, Paratwada, Jittapur, Akot). It was found that the physiologically disordered fruits have low TSS (5.45 to 7.82 o Brix), ascorbic acid (8.43 to 14.97 mg 100 ml -1 ), reducing sugars (1.96 to 2.75%), non-reducing sugars (2.33 to 3.65%) and juice content (34.15 to 47.36%) with more acidity (0.73 to 0.80%), length breadth ratio (1.10 to 1.20), rind thickness (3.57 to 3.98 mm), pomace content (24.68 to 28.35%) and rag content (11.31 to 13.65%) as compared to normal fruits TSS (9.46 to 12.78 o Brix), ascorbic acid (32.15 to 42.57 mg 100 ml -1 ), reducing sugars (3.15 to 4.75%), non-reducing sugars (4.26 to 5.56%) juice content (49.16 to 52.24%), acidity (0.64 to 0.69%), length breadth ratio (0.85 to 0.90), rind thickness (3.57 to 3.98 mm), pomace content (24.68 to 28.35%) and rag content (11.31 to 13.65%) respectively.

Genetic diversity was studied in 14 acid lime genotypes by using morphological markers with the help of Mahanalobis D 2 statistical analysis at All India Co-ordinated Research project on fruits Dr. P.D.K.V., Akola during 2018-19. The genotypes grouped into 3 clusters and maximum number of genotypes was included in cluster I and the cluster II and III having individual each genotype in cluster. The inter cluster distance in all the cases were higher than the intra cluster distance indicating wider genetic diversity among the genotypes of different groups. The highest intra and inter-cluster statistical distance was observed for the cluster I and lowest cluster distance recorded in between cluster II and III. Cluster I have the highest intra cluster distance (1120.04) which included 12 genotypes. The maximum average inter cluster distance was observed in between cluster II and III (4936.37). Lowest average inter cluster distance was between I and II (2346.10). The six axes accounted total variation among acid lime genotypes (92.9%) The characters spreading (E-W) (N-S) (m), ascorbic acid (mg/100ml), plant height (m), average weight of fruits, no. of fruits per plant, juice percentage etc. Showing maximum contribution towards genetic diversity. Magnitude of cluster means for different traits and performance of genotypes of cluster II and III may be considered as parents for hybridization program.

Efforts have been made to prepare dried extracts (solid extract) and extract (aqueous extract) from adulasa leaf. Physicochemical properties of prepared extracts were evaluated. On the basis of their extractable values and alkaloid content (0.30 %) adulasa dried extracts were added at 0.25, 0.35 and 0.45 per cent for preparation of adulasa dried extracts based carbonated RTS beverage. Adulasa was found to be rich in alkaloids. The acceptability of prepared beverage was organoleptically evaluated. The test score indicated that among the carbonated beverages, the beverage with 0.35 per cent adulasa dried extracts with sucralose (0.2 %) as sweeteners was accepted by panel members. TSS of the carbonated beverage was decreased with addition of sucralose further increases with increase in proportion of adulasa dried extracts. Selected carbonated beverage found to be rich alkaloid content (210mg/200ml) and was organoleptically accepted.