Quantitative variables were expressed as mean and standard deviation, and qualitative variables were expressed as absolute and relative frequencies. The correlation between the results of the OAE test and other variables was performed using the chi-square test and calculated by SPSS software version 21.0, with a significance level (p) of 5%. Results: Of the 66 patients included in the study, 45 (68.2%) had used ototoxic drugs and 21 (31.8%) had not. Gentamicin was used for 43 neonates, furosemide by 8 and vancomycin by 6. Among the patients who made use of ototoxic medication, 95.2% had normal OAE test and 2 had absent response (4.8%) (p = 0.4). Conclusion: Positive and negative results in the hearing screening were found both in newborns who used or did not use ototoxic drugs. The isolated use of drug by newborns with absent response in the OAE test was not significant.