This research study intended to provide evidence and proof of the positive effects, that applying of the Bio-Fertilizer Herbagreen produced through nanotechnology has on the agriculture in Albania. There are no doubts that nanotechnology is one of the most relevant innovations not only in agriculture, but also in other scientific fields such as biotechnology, medicine etc. Herbagreen nano-particles are able to directly penetrate plants after being applied, thus becoming immediately part of the plants lifecycle and metabolism. This is due to the new nano-and micro-dimensions of the grinded particles after being treated through TMAC (Tribo-Mechanical Activation of Calcite). This technology does not interfere with the mineral initial composition. It simply grinds the mineral particles in nano-dimensions and by so doing increases sensibly their contact surface. This makes these particles "magically" influence the yield quantity and quality in the treated plants. Based on the experiments performed during two consecutive years on the selected crops of corn and wheat, it was noticed that in addition to a yield increase in the plots treated with this nanotechnology, there was also an increase in the gluten values in wheat, while regarding the percentage values of proteins and lipids it was noted that the values of the treated plots with Herbagreen had the same values of the plots treated with the maximal dosage of chemical fertilizers. It was also concluded, that using of the Herbagreen fertilizers can avoid or reduce the soil and waters contamination caused by only traditional mineral fertilizers use. The financial costs for the treatment can be reduced as well.

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Changes of EUF-extractable nitrogen (N) (nitrate, ammonium, organic N) in 20 arable bare soils, subsequently planted with ryegrass (Lolium multiflorum L.) and cutting three times were investigated in pot experiments. All 20 soils responded qualitatively in the same way. During the period of bare soil, there was a significant increase of EUF-extractable nitrate (EUF NO À 3 ), while extractable ammonium (EUF NH 4 ) remained on the same level and organic N (EUF N org ) decreased. This decrease, however, was not significant. From sowing until the first cutting of the grass, EUF-NO À 3 concentration decreased to almost zero. This low EUF-NO À 3 level was maintained throughout the subsequent experimental period (three cuttings of grass). During the growth of the first cutting, EUF N org decreased while EUF NH 4 remained constant, however, on a low level. EUF NH 4 fell during the growth of the second and third cutting. In this period, how-ever, the N supply of the grass was insufficient. EUF N org decreased during the growth of the second cutting, but increased during the growth of the third cutting. This shows that the EUF-N org fraction represents a transient pool, which gains and loses N. EUF NO À 3 , EUF NH 4 , and EUF N org correlated with the N uptake of the grass. Strongest correlation for EUF NO À 3 was found for the first cutting (p < 0.001), and for EUF NH 4 and EUF N org for the second and third cutting (p < 0.001). Total soil N was not correlated with the N uptake of the grass. EUF N org was only about 2% of the total N. This relatively small EUF-N org fraction, however, is relevant for the mineralization of organic soil N, and the N quantity indicated by EUF N org is in the range of the N amount mineralized in arable soils within a growing season.

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