Gambier (Uncaria gambir Roxb) is one of the sources of natural dyes that able to apply to the textile industry. Textile dyeing industrial wastewater using Gambier comes from several sources. Among them come from the dyeing process itself and from the fixation process wastewater using alum (Al2 (SO4)3), lime (CaO) and tunjung (FeSO4). The wastewater was characterized by high levels of organic pollutants and dyestuffs as well as compositions of fixator raw materials. This study explored the performance of two types of Multi-Soil-Layering (MSL) bioreactors in order to reduce pollutant parameters in such a wastewater. The result revealed that MSL showed good performances. The more MSL layers, the higher the reduction. Reduction of BOD5, COD, and TSS in MSL type 1 could reach 98.6%, 95.3% and 87.1% respectively, while 98.1%, 93.8% and 84.9% in MSL type 2, respectively. The MSL bioreactor could neutralize pH from pH 4.7 to be pH 7.4 and pH 7.1 and able to reduced colour from 427 PtCo to 0.4 PtCo and 1.3 PtCo as well as reduced turbidity from 47 NTU to 0.8 NTU and 1.2 NTU by MSL type 1 and MSL type 2 respectively.
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