Raman spectroscopy is a powerful analytical technique in contemporary medicine and biomedical research due to its exceptional ability to provide an unambiguous spectroscopic signature of the molecular chemical composition, structure...

Raman spectroscopy, an emerging technology for in situ space exploration, has been suggested for life detection for the Europa Lander Mission. However, obtaining spectra of samples from the Europan icy shell requires measurements at temperatures down to -233 °C, which will affect the Raman spectra of any potential biosignatures. In this study, we obtained Raman spectra of amino acids using a 785 nm Raman system at temperatures ranging down to -196 °C, analogous to Europa's surface and near sub-surface. Significant Raman band width narrowing and decreasing variance were observed at lower temperatures leading to higher precision Raman measurements, which required higher spectral resolution that could be as high as 2 cm -1 for full identification of amino acids. Such spectral resolution is much higher than the resolution of contemporary