This research was aimed to identify the influence of Atonic cencentration to the growth of single-node Vanilla cutting planted at various growing media types. Completely randomized design was used in this research with two factors namely Atonic concentration (500 ppm, 1000 ppm, 1500 ppm, 2000 ppm, dan 2500 ppm) and growing media types (tanah, pasir, tanah + pupuk kandang, tanah + pasir, tanah + pasir + pupuk kandang). The result showed that there were no interaction between Atonic concentration and growing media types to the growth of single-node vanilla cutting. Furthermore, the application of Atonic concentration at 2000 ppm established the best result of root length (12,32 cm) and root dry weight (0,16); while the best result of germination percentage (92,23%) revealed by Atonic concentration of 1500 ppm. Regression analysis of germination percentage showed that the optimal concentration of Atonic was 1842 ppm with R² = 60,3%. Mixed media of soil + sand + dung performed the best result in root length, root dry weight and germination percentage.