Background: Hodgkin's disease is a malignant disease in the blood cells, especially the lymphatic ones, and accounts for 30% of the lymphomas. The Epstein-Barr virus (EBV) is associated with a proportion of cases of Hodgkin lymphoma (HL), and this association is believed to be causal. In Iraq, there are very few studies on this subject. Objectives: The purpose of the current study was to determine the association between EBV infection and Hodgkin lymphoma of Iraqi patients. Patients and Methods: Unmatched case-control study was conducted 55 paraffin-embedded tissues of HL of Iraqi patient's in addition to 110 normal noncancerous nasopharyngeal biopsy samples as control group. DNA of EBV was extracted from both controls and neoplastic tissues and analyzed by PCR technique using primers specific to EBV Latent Membrane Protein-1 Oncogene (LAMP-1) for the presence of EBV. Results: This study has shown that EBV were found in 51/55 Hodgkin lymphoma cases (92.73%) and in 85/110 controls (77.27%). This study has identified Gender of HL cases was not risky and not significantly associated with illness ((OR 1.00, CI 0.5220-1.9157, P. Value = 1.000). The results of this investigation show that exposure to EBV was risky and independently associated with HL (OR 3.7500, CI 1.2345-11.3916, P. Value = 0.016). Conclusions :The results do support an association between EBV infection and HL.