IMPF: 01.55Seabuckthorn, a non-leguminous nodule bearing dioecious shrub, is a storehouse of neutraceutical, pharmaceutical and cosmetic usage. The 348 genotypes of Hippophae rhamnoides ssp. turkestanica, H. salicifolia and H. tibetana were collected from 194 locations at 46 major sites across 1,500km from north-east to north-west Himalayas, harboring one of the most harsh, highly variable climatic and ecological conditions and rugged rocky terrain in the range of 3,000?5,000 metres altitude. Amplified fragment length polymorphism (AFLP) and selective amplification of microsatellite polymorphic loci (SAMPL) DNA markers were utilized to assess the genetic diversity of total spectrum of Himalayan germplasm resources and interrelationships among Hippophae taxa. A total of 151, 50, and 41 AFLP loci were detected in Hippophae rhamnoides ssp. turkestanica, H. salicifolia and H. tibetana respectively; of these, 92.6, 30.6 and 25.1% were polymorphic. For SAMPL, the number of markers and polymorphism for these species were respectively, 77 (77.7%), 41 (41.4%), and 23 (23.2%). Nei?s genetic diversity and Shannon?s information index values revealed that populations of H. rhamnoides ssp. turkestanica in Sumur and Raling in Ladakh and Lahaul-Spiti, respectively, were the most diverse. In H. salicifolia and H. tibetana, the populations in Changu, and Takcha and Guling in Spiti valley, were the most diverse. In H. rhamnoides ssp. turkestanica, high levels of interpopulation genetic diversity with little intra-population diversity was accompanied by very low gene flow (Nm) range, estimated by AFLP (0.571?0.943) and SAMPL (0.321?0.726) markers. In H. rhamnoides ssp. turkestanica, the genotypes collected from Uttaranchal were found to be the least diverse. The exclusive characteristics of the nuclear genome in the Uttaranchal genotypes warrant new species rank closer to H. salicifolia rather than to H. rhamnoides ssp. turkestanica. The present results also provide explicit evidence to suggest that both H. salicifolia and H. tibetan deserve species rank.Peer reviewe

Named data networking is one of the recommended architectures for the future of the Internet. In this communication architecture, the content name is used instead of the IP address. To achieve this purpose, a new data structure is added to the nodes of named data networking which is called Pending Interest Table (PIT). Scalability, memory consumption, and integration are the significant challenges in PIT design as it needs to be updated for each packet, and it saves the name of the packet. This paper introduces a new data structure for PIT called DiCuPIT. DiCuPIT is a distributed data structure for the PIT table, that works based on the Cuckoo filter and can cover the three features as above-mentioned. By implementing this PIT, the lookup time shows a 36% reduction compared to the methods based on the Bloom filter and 40% based on hash tables. Moreover, the memory consumption is reduced by 68% compared to the hash tables-based mechanisms and 31% compared to the methods based on the Bloom filter.

-Five accessions of Dipcadi erythraeum, a rare and endemic, bulbous medicinal plant of Thar desert in India, were collected from different districts of Rajasthan (India) to study the intra-specifi c genetic variation existing in their natural populations. To resolve species specifi c cytogenetical mechanism leading to evolution of D . erythraeum, mitotic and meiotic analyses were carried out. The karyotypes of various accessions of D. erythraeum were precisely resolved into ten distinct homologous pairs, not into groups of chromosomes. A striking feature observed meiotically is that bivalents are the most frequent association, while multivalent associations were completely absent in any of the PMCs presently analyzed. Almost diploid like behavior of chromosomes has been the hallmark of the PMCs investigated presently. Overall cytogenetical mechanism underlying the speciation and evolution indicates the probable amphidiploid origin of D. erythraeum.

Fluorescence in situ hybridization with multiple repeated DNA probes applied to the analysis of chromosomal and genomic relationship levels between faba bean (Vicia faba) and its close wild relatives, Caryologia: International Abstract -The organisation of DNA sequences at chromosomal level in twelve Vicia species, more particularly among V. faba and its close wild relatives (V. narbonensis, V. hyaeniscyamus, V. johannis, V. galilaea, V. bithynica), has been performed using fl uorescence in situ hybridization (FISH). The four BamHI discrete size classes of 850, 900, 990 and 1750-bp of highly repetitive DNA isolated from V. faba, and the probes pTa 71 and pTa 794 containing the 18S-5.8S-26S rDNA and 5S rDNA, respectively, were used. The chromosomal location of the two ribosomal gene families distinguished V. faba from its close relatives. FISH to metaphase chromosomes indicates the presence of BamHI family sequences interspersed in both euchromatin and heterochromatin of the V. faba, V. narbonensis, V. hyaeniscyamus, V. galilaea, V. johannis and V. bithynica of taxonomic section Faba, and V. hybrida of section Hypechusa genomes; however, the level of hybridization clearly distinguished the V. faba genome from other species genomes. This analysis provides further evidence that V. faba should be considered as the most distinct. In the context of possible sources of germplasm for V. faba breeding, it is argued that none of the species can be considered as putative allies of faba bean. The repetitive sequences did not hybridize in situ with the chromosomal DNA of fi ve Vicia species of section Vicia and section Cracca. The quantitative variation and/or complete absence of BamHI family repeats enabled differentiation among Vicia genomes.