In this study, two polyproline-derived chiral selectors are bonded to monolithic silica gel columns. In spite of high chiral selector coverage, the derivatization was found to have only a slight effect on the hydrodynamics of the mobile phase through the column. The enantioseparation ability of the resulting chiral monolithic columns was evaluated with a series of structurally diverse racemic test compounds. When compared to analogous bead-based chiral stationary phases, higher enantioseparation and broader application domain were observed for monolithic columns. Moreover, the increase in flow rate produces a minor reduction of resolution, which permits to shorten analysis time. Additionally, increased loadability defines chiral polyproline derived monoliths as adequate for preparative chromatography.

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The increasing significance of chirality in the different fields involving products aimed to interact with biological systems has led to the need to control and produce enantiomerically pure compounds. Chromatography using chiral stationary phases (CSPs) plays an outstanding role in this context. Diverse chromatographic modalities are used in the separation of enantiomers. Among them, enantioselective liquid chromatography is considered at present to be one of the most general methods for the analytical control of the enantiomeric content in samples. Additionally, liquid chromatography is increasingly applied in the purification and isolation of enantiomers from their mixtures. The diversity of CSPs, their characteristics, and their chromatographic conditions of use will be discussed.