Rapid development and advancement of bioresearch at a university's laboratories can have both positive and negative implications for public health and the environment. Many research activities in which biological materials have been created, modified, stored, and manipulated require safety procedures to keep the negative effects on humans and the environment as low as possible. The Occupational Health, Safety and Environmental (OHS&E) Department of the University of Indonesia (UI) is trying to increase the awareness and responsibility of its university members and laboratory staffs who work with biohazard materials by creating a biorisk checklist. The checklist was developed based on WHO guidelines and the National University of Singapore (NUS) Laboratory Manual, which contains 311 questions about the management, administration, and handling of various hazards, recombinant experiments, and animal and plant experiments. A gap analysis was run against the checklist in 14 laboratories at the University of Indonesia Salemba campus, which daily works with highly infectious pathogens and high-risk agents. Overall result showed that none of these laboratories had met all of the checklist items, and there were only 2 laboratories that had implemented more than half of the items. This checklist was proven to be a simple tool for assessing laboratories that handle and store biohazard materials, and it could be used as a monitoring tool for biorisk programs as well. It also could be further developed as a laboratory software application to increase its effectiveness and its accuracy.

Background: Ovarian cancer is one of the leading cancers in women. Seventy percent were founded in the advanced stage, with a 5-year survival rate of only 46%. The current treatment modality is cytoreduction with platinum-based adjuvant chemotherapy as the first line. The effectiveness of chemotherapy is only 60% in the advanced stage, later developing into recurrent. Therefore, additional types of therapy are required based on agents that work specifically in cancer cells and synergize with current standard treatments. This study aimed to know the anti-proliferation effect and apoptosis effect of combination cisplatin with nanocurcumin on SKOV3 cells. Methods: This experimental study was conducted in vitro using the biological cell line SKOV3 to determine the anti-proliferation effect (expression Ki67) and the apoptosis effect (caspase 3 and 8) of combination cisplatin with combination with cisplatin nanocurcumin on the cell. The data were analyzed with unpaired T when regular distribution / Mann Whitney test when the distribution is abnormal and using Graph Pad Prism. Results: Based on this result, 50cc of nanocurcumin is 67 μm, and 50cc of cisplatin is 54 μm, using the MTT Assay method. The viability of the cells in this study decreased according to the dose-dependent, whereas the combined dose of 134 μm nanocurcumin with 108 μm cisplatin found the lowest life cell, 24.3% (p <0.001). Ki67 expression was low in the SKOV3 cells post-exposure to cisplatin, but increased post-exposure to nanocurcumin, suspected nanocurcumin at certain doses of pro-oxidant properties that triggered proliferation. Caspase 3 and 8 cannot be detected in this cell by the ELISA method. Conclusions: Nanocurcumin has a potential effect on chemosensitive cells. However, our study shows no healing effect in chemoresistance cells, particularly SKOV3.

Diabetic keratopathy is one of the most common ocular complications in diabetes mellitus. Protocol for diabetes induction in rat model also has been established in many centers. Nonetheless, method in developing diabetic keratopathy rat model has not been well covered. Streptozotocin (STZ)-induced diabetes is widely being used as animal diabetic model. The purpose of this study is to obtain an animal model of diabetic keratopathy that can be used to study the morphology, metabolism, and function of cornea in cases where human samples can be difficult to obtain.A single dose STZ (50 mg/kg) was injected intraperitoneally to control and intervention group. Plasma glucose level concentrations were tested in day 3 post injections. Obtained animal model of diabetic keratopathy, with significant difference of blood glucose level between intervention and control group (P<0.00). Sensibility of cornea was decreased by week 14 th in intervention group. Epithelial defect were more prominent in diabetic group. Despite the differences between human and animal characteristic of diabetic keratopathy, the use of animal models has contributed to better understanding of this disease and to examine more effective treatment.

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Rapid progress of technologies does not only exclusively belong to developed countries nowadays but also spread out to developing countries including Indonesia. There are many Indonesian universities and emerging translational medicine centers that had declared themselves as research centers focusing on to bring science from bench to bedside. Since the outbreak of 2007 Avian Influenza, Indonesia had been starting to accumulate more knowledge and experiences related to biosafety and biosecurity. While Indonesian researchers were focusing on biorisk management of high pathogenic bioagents, students were also being exposed gradually to more sophisticated biological hazards through the utilization of synthetic biology and genetic modifications on their own researches. Nurturing the responsible future generation of scientist whom aware of the ethical, biosafety, biosecurity concerns cannot become more important, considering the possibility of dual use research results, which could bring either prosperity or chaos to the universe.