The phenomena of aestivation and overwintering of ants present a most interesting study. The similarities between the two as well as their many differences offer excellent comparisons. Little work has actually been accomplished with ants in this field in spite of the studies of arthropod hibernation instigated by Holmquist (I) and others. The purpose of this paper is to present some of the more important physical and biotic factors involved in the aestivation and overwintering periods of the Occident ant. Pogonomyrmex occidentalis Cresson, in a portion of the Northern Desert Shrub region, near Twin Falls, Idaho.
Several samples of commercial grade honey collected from different parts of tennessee during the summer of 1973 were analyzed for chlorinated hydrocarbon insecticide (CHI) residues. A "Modified Mill's Procedure" was used to cleanup the samples prior to gas chromatographic analysis using electron capture (EC) detection. The presence of CHI residues was confirmed by analysis on three different columns of widely varying polarity. Most of the samples contained CHI residues at 0.01-0.30 parts per billion (ppb) level. Beeswax produced during the same season contained several times higher levels of the residue than the honey samples. Recoveries of CHI residues varied from 81-95 percent by the procedure employed.
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