Introduction: For diagnosis, sub-categorization and follow up of Acute Leukemia (AL), phenotypic analysis using flow cytometry is mandatory.
Material and methods: We retrospectively analyzed immunophenotypic data along with cytogenetics/molecular genetics data (wherever available) from 631 consecutive cases of AL diagnosed at our flow cytometry laboratory from January 2014 to August 2017.
Results: Of the total 631 cases, 52.9% (n=334) were acute lymphoblastic leukemia (ALL), 43.9% (n=277) acute myeloid leukemia (AML), 2.2% (n=14) mixed phenotypic acute leukemia (MPAL) and0.5% (n=3) each of acute undifferentiated leukemia (AUL) and chronic myeloid leukemia in blast crisis (CML-BC). ALL cases comprised of 81.7% (n=273/334) B-cell ALLs (95.2%, n=260/273 common B-ALLs and 4.8%, n=13/273 Pro B-ALLs). CD13 was the commonest cross lineage antigen expressed in B-ALL (25.6%), followed by CD33 (17.9%) and combined CD13/CD33 (11.3%) expression. T-ALLs constituted 18.3% (n=61/334) of total ALLs and included 27.9% (n=17) cortical T- ALLs. CD13 was commonest (32.7%) aberrantly expressed antigen in T-ALLs, followed by CD117 (16.0%). AML cases included 32.1% (n=89/277) AML with recurrent genetic abnormalities, 9.0% (n=25/277) with FLT3/NPM1c mutation and 58.9% (n=163/277) AML NOS including 14.7% (n=24/163) AML M4/M5, 1.8% (n=3/163) AML M6 and 3.7% (n=6/163) AML M7. In AMLs, CD19 aberrancy was the most common (16.3%) followed by CD7 (11.9%).
Conclusion: In this study we document the spectrum; correlate the immunophenotype with genetic data of all leukemias, especially with respect to T-ALL where the data from India is scarce.
compression. All lymphatic connections to the kidneys Case report and ureters were ligated and divided. Biopsies were taken from the lymphatic tissue. An 18-year-old man presented with a complaint of passing milky urine of 3 months' duration. Despite a Post-operatively, the urine was clear and no chylomicrons were detected. The patient was discharged after 2 normal appetite, he had severe weight loss (35 kg). He had no previous history of filariasis or tuberculosis and weeks with normal serum sodium and improved protein levels. The histology showed adipose tissue containing was not from an area endemic for filariasis. Clinical examination was unremarkable except for extreme small lymph nodes, sinusoidal dilatation and numerous dilated vascular channels with irregularly organized emaciation and pedal oedema. Serum biochemistry revealed normal renal function, hyponatraemia and smooth muscle in their walls. Some of these channels were continuous with the peripheral sinus of the lymph hypoproteinemia. The urine was positive for chylomicrons (20 g/L) and the 24 h urinary protein excretion node (Fig. 2); this was consistent with a cystic lymphangioma. was 9.8 g. An IVU showed right-sided hydronephrosis; the right ureter was not visible. CT showed a hyperdense lesion in the retroperitoneum extending from the pelvic
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