Aim The aim of this in-vitro study is to compare the antibacterial efficacy of Mineral trioxide aggregate (MTA) and Biodentin along with the incorporation of silver and Zinc oxide Nano particles. Materials and methodology Antimicrobial effect of MTA and Biodentin impregnated with silver and Zinc oxide Nano particles were tested against Enterococcus faecalis, Lactobacillus and Candida albicans. The minimal inhibitory concentration of the following materials was measured and the antimicrobial effect was determined bothquantitatively and qualitatively by using Brothdilution method and membrane enclosed immersion test . The data’s were statistically analyzed using SPSS 21 IBM software and comparison between the two groups in normal data distribution were done using Student’s t-test. Results Test indicates that the antimicrobial activity of Biodentine with silver nano particles showed very strong antibacterial action compared with other materials to the three bacterial strains followed by MTA and Silver Nano particles. There was a statistically significant difference between these materials Conclusion By quantifying the amount of nanoparticles that can be added to the bioactive materials These can be successfully used in the clinical practice.