Thirty lactating ewes were used to evaluate the effect of superovulation on milk production. Twelve ewes had been injected, prior to mating, with 700 IU of pregnant mare serum gonadotropin; 18 ewes were injected with saline as a control. Thirteen ewes (nine control and four superovulated ewes) were fed at low plane of nutrition; the other ewes (nine control and eight superovulated ewes) were fed at high plane of nutrition. Superovulated ewes, fed at both low and high planes of nutrition, had dramatically higher milk yields (59%), and their milk composition was not changed. Plane of nutrition increased milk lactose and P contents without significant effect on milk production. The increased milk yields in the superovulated ewes were accompanied by increases in dry matter, gross energy intakes, and gross efficiency of milk synthesis. At the end of lactation, superovulated ewes had higher mammary dry fat-free tissue, total DNA, and total RNA. The results demonstrated that superovulation prior to mating dramatically increased milk production and efficiency regardless of plane of nutrition. Increased milk production and efficiency in the superovulated ewes were due to the increased mammary secretory cell numbers and their synthetic activities presumably through the increased endogenous hormonal stimulation of mammary growth and development during pregnancy.
Spleen is one of the important lymphoid organs with wide variations of morphological and physiological functions according to species. Morphology and function of the spleen in bats, which are hosts to several viral strains without exhibiting clinical symptoms, remain to be fully elucidated. This study aims to examine the spleen morphology of fruit bats associated with their physiological functions. Spleen histological observations were performed in three fruit bats species: Cynopterus titthaecheilus (n = 9), Rousettus leschenaultii (n = 3) and Pteropus vampyrus (n = 3). The spleens of these fruit bats were surrounded by a thin capsule. Red pulp consisted of splenic cord and wide vascular space filled with blood. Ellipsoids in all three studied species were found numerously and adjacent to one another forming macrophages aggregates. White pulp consisted of periarteriolar lymphoid sheaths (PALS), lymphoid follicles and marginal zone. The lymphoid follicle contained a germinal centre and a tingible body macrophage that might reflect an active immune system. The marginal zone was prominent and well developed. This study reports some differences in spleen structure of fruit bats compared to other bat species previously reported and discusses possible physiological implications of the spleen based on its morphology. K E Y W O R D S flying foxes, histology, immunology, morphology, physiology, viral reservoir
ABSTRAKTestosteron merupakan salah satu hormon anabolik yang dapat memacu pertumbuhan massa otot dan tulang. Hormon testosteron mampu merangsang sekresi growth hormone yang berperan dalam pertumbuhan. Penelitian ini bertujuan untuk mempelajari pengaruh pemberian hormon testosteron dengan dosis bertingkat terhadap performa dan kecernaan pakan ayam broiler. Sebanyak 44 ekor ayam broiler berumur 15 hari dengan rata-rata bobot badan antara 650 ± 71 g dibagi menjadi 4 kelompok (K, T 1 , T 2 , dan T 3 ). K adalah kelompok kontrol; T 1 , T 2 , dan T 3 adalah kelompok yang diberi testosteron dosis 1, 2, dan 4 mg per ekor. Parameter yang diukur adalah bobot badan, konsumsi pakan, rasio konversi pakan, serta persentase kandungan nutrisi (di dalam pakan dan feses). Hasil penelitian menunjukkan pemberian testosteron dosis 1 dan 4 mg dapat meningkatkan pertambahan bobot badan harian secara signifikan (p<0,05) pada ayam broiler yang berumur antara 15 dan 18 hari. Testosteron dosis 2 mg meningkatkan bobot badan harian secara signifikan (p<0,05) pada ayam broiler yang berumur antara 21 dan 24 hari. Konsumsi pakan, rasio konversi pakan, dan kecernaan pakan ayam broiler yang diberi hormon testosteron tidak menunjukkan perbedaan dibanding dengan kontrol. Akan tetapi, pada kelompok yang diberi testosteron dosis 4 mg, nilai kecernaan lemak, protein, dan karbohidrat cenderung meningkat.Kata kunci: broiler, kecernaan, pertambahan bobot badan harian, proksimat, testosteron ABSTRACTTestosterone is one of the anabolic hormone that can trigger the growth of muscle mass and bone. Testosterone hormone can stimulate secretion of growth hormone that has a role in growth. The aim of this research was to observe the administration of multi-dose testosterone hormone on broiler chickens performance and feed digestibility. Fourty four broiler chickens 15 days old with average weight 650 ± 71 g were divided into 4 groups (K, T 1 , T 2 , and T 3 ). K was control group; T 1 , T 2 , and T 3 were groups which given testosterone dose 1, 2, and 4 mg each chicken. Parameters measured were body weight, feed consumption, feed conversion ratio (FCR), and nutritions percentage (in feed and feces). The results showed that testosterone dose 1 and 4 mg could increase the daily body weight gain significantly (p<0.05) in broiler chickens aged between 15 and 18 days old. Testosterone dose 2 mg could increase the daily body weight gain significantly (p<0.05) in broiler chickens aged between 21 and 24 days old. Feed consumption, FCR, and feed digestibility of chickens given testosterone did not show any difference compare with control. However, in group which given testosterone dose 4 mg, the digestibility values of fat, protein, and carbohydrates tended to increase.
Pada penelitian ini, purwoceng diberikan pada tikus betina bunting umur 1-13 hari dengan tujuan mengetahui bobot badan, ovarium, dan uterus serta mempertahankan titik implantasi yang terbentuk sebelum masa implantasi. Penelitian ini menggunakan 2 kelompok yaitu kelompok tikus bunting kontrol dan tikus bunting yang mendapatkan purwoceng dengan dosis 25 mg/300 g bobot badan. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi purwoceng cenderung memiliki bobot ovarium dan uterus lebih berat dibandingkan kelompok kontrol. Purwoceng juga menyebabkan jumlah titik implantasi tikus hampir mendekati jumlah korpus luteum yang sudah terbentuk, dan ini menunjukkan bahwa keberhasilan implantasi lebih baik pada kelompok tikus yang diberi purwoceng.
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