The specific roles of nutrients in reproduction are not well defined in sheep. Hence, the relationships between certain mineral (Ca, Mg) and element (Cu, Fe, Mn, Zn) levels at mating and pregnancy rates was investigated in fat-tailed Morkaraman ewes synchronised with different hormones (melatonin, norgestomet implant and eCG) early in anoestrus season. For this purpose, 40 healthy ewes (aged 2-4 years) allocated in the synchronised (n=30) and no-treated group (n=10) were used during mid-May (suckling period). On the eCG injection day, fertile rams (n=8) were introduced into the groups and, then the oestrus signs were visually monitored twice daily for 7 days. The oestrous ewes were allowed to handmate using rotational (four rams daily) mating system. Additionally, jugular blood samples were collected on the days of onset of oestrus (before mating) for determination of serum Ca, Cu, Fe, Mg, Mn and Zn levels. Presence of pregnancies was diagnosed by real-time ultrasonography 35 days post-mating. Totally, 25 ewes became pregnant (21 ewes with stimulatedand 4 with spontaneous oestrus), while 9 ewes (all received stimulation) were not pregnant. In non-pregnant ewes, unlike other minerals and elements, the Cu and Zn values were significantly lower than their reference values (for Cu: 0.87±0.16 mg/dl vs. 9.20 mg/dl; for Zn: 2.06±0.30 g/dl vs. 3.8 g/dl, resp.; P<0.01 in both cases). In pregnant ewes, the corresponding values remained unchanged (P>0.05). Hence, the present findings indicate that the low Cu and Zn levels during the oestrus might adversely affect the subsequent pregnancy rate in suckling ewes.

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In this study was investigated effects of borax (BX) on inflammation markers, haematolojical parameters and total oxidant (TOS)-antioxidant status (TAS) in rats applied 3-methylcholanthrene (3-MC). In this research a total of 24 Wistar Albino rats were used. They were divided into 4 groups each containing 6 rats. 1st group was separated as a control group. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose to the 2nd group with i.p. way. BX was given to 3rd group 300mg/L/day dose with drinking water during 150 days. 3-MC was applied twice a week first 2 weeks 25 mg/kg dose with i.p. way and BX were given with drinking water during 150 days to 4th group. At the end of the study blood analysis, tumor necrosis factor alpha (TNF-α) and interleukin 1 beta (IL-1β) levels in 3-MC group; TOS and oxidative stress index (OSI), platelet (PLT) levels in 3-MC and 3-MC+BX groups showed significantly increases when compared to other groups. It was determined that lymphocytes % (LY%) of ever 3 groups were significantly higher; however, neutrophil % (NEU%) were significantly fewer according to control group. Haemoglobin (HGB) and hematocrit (HCT) values of 3-MC+BX groups showed significantly decrease according to other groups (P≤0.05). Mean corpusculer volume (MCV) in 3-MC and 3-MC+BX groups showed significantly decrease when compared to other groups (P≤0.05). As a result, in case of exposure to 3-MC, long-term use of BX with oral ways may not decrease oxidative stress, may changes haematolojical parameters such as, WBC, LY%, NEU%, PLT, HGB, HCT, MCV. However, these changes remain within physiological limits. Even so, in the use of BX should be considered use of iron. Furthermore, BX with the abovementioned dosage may be used to reduce the levels of TNF-α, IL-1β, IL-6 being inflammation and cancer markers. Borax, Haematology, Inflammation, Interleukin, Rat, Tas, Tos, Keywords: 3-Metilkolatren Uygulanan Sıçanlarda Boraksın İnflamasyon, Hematolojik Parametreler ve Total Oksidan-Antioksidan Durumlar Üzerine Etkileri ÖzetBu çalışmada, 3-metilkolatren (3-MC) uygulanan sıçanlarda boraksın (BX) inflamasyon göstergeleri, hematolojik parametreler ve total oksidan (TOS)-antioksidan durumlar (TAS) üzerine etkileri araştırıldı. Çalışmada toplam 24 Wistar Albino sıçan kullanıldı. Sıçanlar her grupta 6'şar adet olacak şekilde 4 gruba ayrıldı. Birinci Grup kontrol grubu olarak ayrıldı. İkinci gruba 25 mg/kg dozunda haftada iki kez ilk 2 hafta 3-MC i.p. yolla uygulandı. Üçüncü gruba BX 300 mg/L/gün dozunda içme suları ile 150 gün boyunca verildi. Dördüncü gruba 3-MC 25 mg/kg dozunda haftada iki kez ilk 2 hafta i.p. yolla uygulandı ve BX 300 mg/L/gün dozunda içme suları ile 150 gün boyunca verildi. Çalışma sonunda kan analizlerinde, diğer gruplarla karşılaştırıldığında 3-MC grubunda tümör nekrozis faktör alfa (TNF-α) ve interlökin 1 beta (IL-1β); 3-MC ve 3-MC+BX gruplarında ise, TOS, oksidatif stres indeksi (OSI) ve trombosit (PLT) seviyeleri istatistiksel önemde artış gösterdi. Kontrol grubuna göre her 3 gruptaki % lenfosit (%LY...

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