This study was purposed for confirming detection and typing of Anaplasma spp in infected sheep from Al-Diwaniyah province, Iraq. Sampling of 50 blood specimens was performed from clinically-identified infection of anaplasmosis. The samples of the blood were subjected to DNA extraction followed by polymerase-chain-reaction (PCR)-based detection of the Anaplasma marginale using major surface protein (MSP4) gene. The results have shown that 8 blood samples were infected with A. marginale. The PCR-based identification has revealed a confirmative identification of the Anaplasma marginale in the infected sheep. The study identifies Anaplasma marginale as a member of infectious agents that affect sheep in the study city.

This study aimed to diagnose orf virus ORFV from infected goats in Al-Qadisiyah Governorate of Iraq, using polymerase chain reaction and construction of phylogenetic tree. Clinical examination was made on 400 goats in different herds and crusty scabs were collected from forty infected goats. All scabs were subjected to (PCR), sequencing, and phylogenetic analysis involving the presence of GIF gene GM-CSF inhibitor factor, a characteristic gene of the ORFV. The typical proliferative skin lesion with overall orf prevalence results by PCR was 10% with low mortality rates and high significance variation with age and gender. Two positive PCR amplicons were sent for partial-genebased sequencing and Phylogenetic analysis. Multiple alignments of sequences presented close identities with two Iraqi global isolates of the virus. This study didn't only report novel sequences from the local orf virus isolates, but it also provides important data about transmission of local sheep orf virus into local goat during closely rearing and grazing and orf outbreak in small ruminant in Iraq might be suspected.