Introduction: Now a day, antibiotic-resistant bacteria are increasing and became a medical worldwide problem, therefore the achievement of a new safe disinfectant is deemed necessary. Pimpinella anisum (Pa) is an aromatic plant, belonging to the Umbelliferae family. It is a well-known traditional medicinal herb that has been used in old medicine as an antibacterial, anticancer, antiulcer, therefore, the aim of this study is evaluation of the anti-bacterial effect of PA extracts against Probiotics and pathogens bacteria. Method: 96-well microplates MICs were determined by the broth microdilution method. Five Serial dilutions from 50 to 1 μg/mL concentrations were admitted for all bacteria which include: Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and Probiotics complex. Results: PA watery extract demonstrates a statically inhibitory effect in just 50 g/L concentration against E.Coli, E. Faecalis, and Probiotic p= 0.000 for all, whereas this concentration was 25 g/L for Staph. Aureus and Pu p=0.000 and p= 0.007 respectively. PA alcohol extract displays the same effect in 1g/L for all bacteria p= 0.000 for all bacteria. Conclusion: Our results showed that PA in both watery and Alcohol extractions can inhibit both pathogenic and non-pathogen bacteria whereas active compounds are alcohol soluble. Long-time consumption of PA in an herbal product could disrupt normal bacteria of the gastrointestinal system while PA watery extract can promote Probiotics.

Introduction: Herbs of the lavender genus are widely used today as anti-bacterial drugs in traditional medicine. Lavandula angustifolia (LA) is endemic in the Iberian Peninsula and common in Iran that belongs to the Lamiaceae family. Lavandula oils, well known for their scent and aroma, have been used in the perfumery and food industry for many years, therefore the aim of this study is the assessment of the anti-bacterial effect of LA extracts against pathogens and non-pathogen bacteria. Methods: 96-well microplates MICs were determined by the broth microdilution method. Five Serial dilutions from 50 to 1 μg/mL concentrations were admitted for all bacteria which include: Escherichia Coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853 and Staphylococcus aureus ATCC 25923, and Probiotics complex. Results: LA watery extract demonstrate the statically inhibitory effect in just 50 g/L concentration against E.Coli, E. Faecalis, Pu, and Probiotic p= 0.024, 0.025, 0.004, and 0.012 respectively, whereas this concentration was 1g/L for Staph. aureus p=0.026. LA alcohol extracts display the same effect in 1g/L for all bacteria p= 0.000.Conclusion: Our results showed that LA in both watery and Alcohol extractions can inhibit both pathogenic and non-pathogen bacteria whereas active compounds are alcohol soluble. Long-time consumption of LA in the herbal product could disrupt normal bacteria of the gastrointestinal system.