The condensation of phthalic anhydride afforded structurally modified isoindoline-1,3-dione derivatives with selected amino-containing compounds. The title compounds ( 2 – 30 ) have been characterized by thin-layer chromatography (TLC), infrared spectroscopy, 1 H and 13 C NMR spectroscopy, and mass spectroscopy. All of the compounds were assessed for their antimycobacterial activity toward the H37Rv strain by a dual read-out assay method. Among the synthesized compounds, compound 27 possessed a significant IC 50 of 18 μM, making it the most potent compound of the series. The InhA inhibitory (IC 50 ) activity of compound 27 was 8.65 μM in comparison to Triclosan (1.32 μM). Computational studies like density functional theory (DFT) study, molecular docking, and dynamic simulation studies illustrated the reactivity and stability of the synthesized compounds as InhA inhibitors. A quantum-mechanics-based DFT study was carried out to investigate the molecular and electronic properties, reactivities, and nature of bonding present in the synthesized compounds and theoretical vibrational (IR) and isotropic value ( 1 H and 13 C NMR) calculations.

Research and teaching have an array of unique hazards that reflects both the variety and continuous evaluation of their operation. These include technical, physical, chemical, or biological hazards. We are reporting a laboratory accident involving acryloyl chloride (chemical hazard), its consequences, safety precautions, and the lesson learned from this incident. Acryloyl chloride is a highly toxic and volatile liquid. After being accidentally exposed to acryloyl chloride, a victim experienced blackout, headache, dizziness, tiredness, nose bleeding, persistent burning of the eyes, and intense nausea and vomiting. A victim reported two distinct hazardous effects, namely, nose bleeding and stomach ulcers, which were not mentioned in the SDS of acryloyl chloride. To avoid further exacerbation of consequences of acryloyl chloride accident, it may be preferable to initiate steroidal therapy along with symptomatic treatment from the start. The accidental consequences and lessons learned from this tragedy will serve as guiding factors for research scholars, postdoctoral fellows, principal investigators (PI), safety professionals, institutions, occupational health nurses, physicians, and toxicologists to prevent anything similar from happening again in the future.

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