Older age is very closely correlated to risk for developing type 2 diabetes. In the present study we evaluated the effect of age and gender on biochemical, hormones and adipocytakines parameters: Fasting blood sugar, HbA1c, insulin, Insulin resistance, Blood urea, serum creatinine, calcium, phosphorus, parathyroid hormone, calcitonin, vitamin D, adiponectin and tumor necrosis factor-α. Eighty random samples of type 2diabetes patients and a control group which included 20 healthy subjects. The average age was 45.18 ± 1.18year. The blood was collected for study the biochemical, hormones and adipocytakines parameters. The results: The age of all patients was 45.18 ± 1.18year compared with the control 38.56 ± 2.21year showed significant (P<0.05) differences between them. With gender according to no significant differences were found between male 51.95% and female 48.05% in patients' group and control 55.56%, 44.44% respectively. The finding showed increasing levels of FBS with increasing age. The lowest level of FBS was in 20-29 year, significantly (P<0.05) lower than the other age categories, increasing levels of HbA1c was found with increasing age. Serum insulin and IR levels revealed a significant (P<0.05) increase in 40-49 and 50-59 years. Increasing levels of Blood urea with increasing age. Parathyroid hormone and vitamin D were significantly (P<0.05) increased in age category 20-29 year. Increase in levels of FBS, Blood urea, Serum creatinine and calcitonin levels in men than women. The results of the present study showed the impact of type 2 diabetes on age and gender.