Background: Amongst all of the biarticular muscles of the human body hamstring have a greater tendency to shorten. Increased tension in the neural structures has also been shown to be one of the most important causative factor for the hamstring tightness for which mobilisation of the nervous system has emerged as a significant adjunct apart from routine stretching techniques. There are lots of studies available which has proven the effect of stretching on the hamstring flexibility, but only after giving the multiple sessions. Thus the aim of this study was to determine whether a single session of hamstring stretching by neural slump and static stretch technique improves hamstring flexibility or not.Materials and Methods: 60 healthy individuals between the age groups of 18-30 as per the inclusion and exclusion criterion were enrolled for the study. Hamstring tightness was measured using knee flexion angle. Subjects were asked to actively extend their knee from 90-90 hip knee flexion in supine position, individuals having knee flexion angle more than 15 degrees were assumed to have hamstring tightness and were randomly allocated and divided into 2 groups, group A was given static stretch in modified Hurdler's position and group B was given neural slump stretch, having 30 subjects in each group.Result: Data was collected and statistically analyzed using the appropriate statistical tests. Results showed significant reduction in hamstring tightness in both the groups and on comparison, group B (Neural slump stretch) was found to be more effective than group A (Static stretch).Conclusion: Neural slump stretch was found to be more effective in immediately improving the hamstring flexibility as compared to static stretch.