Background: Professional quality of life is the quality that everyone feels in relation to their work. Both the positive and negative aspects of doing a job influence the professional quality of life. The majority of people spend more time at work than they do anywhere else, doing anything else. Objectives: The aim of this research was to study the relationship between professional quality of life and general health in the staffs of Rajaee trauma hospital. Methods: This was a cross-sectional study, which was conducted at Rajaee trauma hospital of Shiraz during a 12-month period (2014). Participants were selected by systematic random sampling. Results: We included 331 staff with mean age of 29.32 (5.1) years, amongst who there were 119 (36%) males and 212 (64%) females. Gender, marital status and education did not affect professional quality of life and general health, but emergency department personnel compared to other sectors had higher prevalence of depression and traumatic stress. In addition, the staffs were in good situation in terms of burnout, secondary traumatic stress, physical dysfunction, social dysfunction and severe depression and in moderate level in terms of compassion satisfaction and anxiety and sleep disorders. Conclusions:The study findings confirmed a significant relationship between professional quality of life (ProQol) and general health questionnaire (GHQ)-28 dimensions. Reducing burnout and secondary traumatic stress as well as increasing compassion satisfaction had a direct impact on decrease in mental health problems in the staff.