In recent decades, reproductive problems were raised alongside the continuous genetic selection for performance purposes in poultry. As such, a negative correlation was observed between reproductive and production performance, which may possibly imply that the high growth rate in poultry causes physiological disorders such as decreased libido and frequency of mating. A study was conducted to establish the efficacy of feeding graded levels of camphor on reproductive function of Japanese quail as a bird model. Two-hundred-and-eighty one-day-old quail chicks were randomly assigned to individual cages in 7 treatments (zero [control], 250, 500, 750, 1,000, 5,000, or 10,000 ppm of camphor). The results demonstrated that testosterone concentration displayed an uncertain attitude, as the lower dosages of camphor used (250, 500, and 750 ppm) had the highest values. Thyroxine (T) linearly increased with increasing camphor levels in male birds (P < 0.05). Triiodothyronine (T) also was increased in the groups receiving a camphor-containing diet in comparison with the control (P < 0.05). The male birds' libido behavior was linearly increased with the increase of camphor concentration (P < 0.05). Dietary camphor in 500 and 10,000 ppm caused the highest percentage of fertility (P < 0.0001) and hatchability (P = 0.0117), respectively. As well, the groups with 5,000 and 10,000 ppm of camphor accounted for the highest percentage of hatched-fertile eggs (P = 0.0055). The highest rate of sperm penetration in the yolk inner perivitelline layer (IPVL) was demonstrated by the group allotted with 10,000 ppm camphor (P < 0.05). This group also had the highest hole persistency in IPVL. Conclusively, the result indicated that camphor can be added in poultry diet for the improvement of reproductive functions.

This study aimed to investigate the effect of supplemental camphor on the performance and immune functions of Japanese quail by feeding graded levels (0 (control), 250, 500, 750, 1000, 5000 or 10 000 ppm) of camphor during a 42-day feeding trial. In all, 280 1-day-old quail chicks were randomly assigned into 28 cages of 10 chicks each with separate feeders. The results clearly demonstrated that camphor did not have a significant effect on BW, BW gain, total experimental average daily feed intake, feed conversion ratio, internal organ relative weights and biochemical parameters such as uric acid, albumin, total protein and triglyceride; however, plasma cholesterol concentration was significantly different in a linear manner, in which 500 ppm of camphor resulted in a lower level of cholesterol. Alternatively, greater concentrations of glucose and high-density lipoprotein (HDL) were also found in 5000 and 1000 ppm of camphor groups, respectively. Cellular responses to the phytohaemagglutinin-P and 2,4-dinitro 1-chlorobenzene skin test were not influenced by dietary camphor. Humoral responses to secondary sheep red blood cells, avian influenza virus (AIV) and Newcastle disease virus (NDV) immunisations were positively influenced by camphor supplementation, in which greater secondary response to sheep erythrocytes belonged to 750 and 1000 ppm of camphor groups; whereas, diet supplementation with camphor had no significant effect on lymphoid organ weights and heterophil-to-lymphocyte ratio. The greatest AIV antibody titers were seen in groups, which received 1000 and 5000 ppm of camphor (P<0.05) and the values of NDV antibody titers increased with an increase in the camphor consumption. Furthermore, dietary inclusion of 500 ppm of camphor positively decreased coliform populations in the gastrointestinal tract (GIT). In addition, an increase in lactic acid bacteria was also observed in quails, which were fed on the diets containing 750 ppm camphor. Collectively, these data suggest that as a phytogenic feed additive, camphor may effectively act as a modulator of health status (by increasing glucose and HDL), GIT microbiota and immunological responses of the Japanese quail.

The unprecedented rate of global warming requires more immaculate strategies to fight the heat stress and its detrimental effects on poultry sector. Nutritional strategies, particularly herbal extracts, play a crucial importance in this scenario. Therefore, this study was conducted to evaluate the effects of a novel selected mixture (hereafter refers to as Stress-Bio-Max, SBM ) comprising St John's wort, lavender, Melissa officinalis extracts, betaine, and Vit C on heat-stressed broilers. A total of 300 male broiler chicks were allocated to 4 treatments: T1, Control (no SBM in drinking water); T2, 0.25 mL/L of SBM in drinking water; T3, 0.5 and T4, 1 mL/L of SBM, which applied since d 32. The birds underwent heat stress (40 ± 1) daily from d 35 to 42 of the experiment (2 pm–8 pm). The results show the superior weight gain of T2 and T3 from d 7 to 42 compared to other groups, 72.5 and 72.7 g/d, respectively ( P < 0.05). Corticosterone level was higher on d 35 in T1 (4.674 ng/mL, P < 0.05) and lower in T2 and T3 (2.64 and 2.952 ng/mL, respectively, P < 0.05); T2 and T3 also caused the lowest concentrations of corticosterone on d 40 (5.198 and 6.458 ng/mL, respectively, P < 0.05). The superior triiodothyronine levels belonged to T2 (0.935 ng/mL, P < 0.05) on d 35 and T2 and T3 on d 40 (0.699 and 0.582 ng/mL, respectively, P < 0.05). T2, T3, and T4 caused a rise in glutathione peroxidase ( GPX ) and superoxide dismutase ( SOD ) activities ( P < 0.05). A significant higher amount of total antioxidant capacity ( TAC ) belonged to the groups treating with SBM ( P < 0.05). The SBM in T2 and T3 resulted in the lowest levels of malondialdehyde ( MDA ), aspartate transaminase ( AST ), alanine aminotransferase ( ALT ), and alkaline phosphatase ( ALP ). Results from this study indicate that SBM may alleviate the negative impacts associated with heat stress in broiler chickens.

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