The defense strategies of plants against pathogen attacks included more than two kinds such as biochemical reactions, structural, hormonal reactions, and elicitors. On the other hand, pathogens in general have different ways and strategies that facilitate their infections and make them successful. Continuously, the interaction between tomato plants and root-knot nematodes causes many reactions involving both morphological symptoms and genetically on the molecular level. However, fundamental aspects of hormonal influences studies on plant nematode interactions are still complicated especially on the level of molecular genetics. Precise recognition of tomato-induced proteins is necessary for identifying plant-induced systemic resistance (ISR). In this paper, the target is the recognition of the protein profile changes accompanied by the biological changes through both processes of infection of two tomato cultivars (resistance and sensitive) with root-knot nematode and treated with two different concentrations of SA 0.1 and 5 mM. The results confirmed that induction of tomato roots infected with root-knot nematodes (RNK) and treated with Salicylic acid (SA) was significant especially in 5mM concentration of SA that appeared to have an excess of intensive bands in sensitive and resistant cultivars as compared to control roots. On the other hand, the bands were light in 0.1 mM concentration of SA.