Periphyton is an important component of stream bioassessment, yet methods for quantifying periphyton biomass can differ substantially. A case study within the Arkansas Ozarks is presented to demonstrate the potential for linking chlorophyll-a (chl-a) and ash-free dry mass (AFDM) data sets amassed using two frequently used periphyton sampling protocols. Method A involved collecting periphyton from a known area on the top surface of variably sized rocks gathered from relatively swift-velocity riffles without discerning canopy cover. Method B involved collecting periphyton from the entire top surface of cobbles systematically gathered from riffle-run habitat where canopy cover was intentionally avoided. Chl-a and AFDM measurements were not different between methods (p = 0.123 and p = 0.550, respectively), and there was no interaction between method and time in the repeated measures structure of the study. However, significantly different seasonal distinctions were observed for chl-a and AFDM from all streams when data from the methods were combined (p < 0.001 and p = 0.012, respectively), with greater mean biomass in the cooler sampling months. Seasonal trends were likely the indirect results of varying temperatures. Although the size and range of this study were small, results suggest data sets collected using different methods may effectively be used together with some minor considerations due to potential confounding factors. This study provides motivation for the continued investigation of combining data sets derived from multiple methods of data collection, which could be useful in stream bioassessment and particularly important for the development of regional stream nutrient criteria for the southern Ozarks.

Watershed managers generally focus on P reduction strategies to combat freshwater eutrophication despite evidence that N co‐limits primary production. Our objective was to test the role of P in limiting stream periphyton biomass within the Buffalo River watershed in Arkansas by conducting a 31‐d streamside mesocosm experiment. To represent potentially different starting states, cobbles were transplanted from two different tributary streams and initially exposed to a range of P (0, 0.012, 0.025, 0.05, 0.1, and 0.2 mg L−1 P) to assess benthic ash‐free dry mass (AFDM) and chlorophyll‐a (chl a) and responses during a P only enrichment period. Later, the experiment was continued under a N/P (10:1 molar ratio) enrichment gradient to examine co‐limitation. Mean AFDM was higher on Day 31 of the N+P enrichment compared with Day 17 of the P‐only enrichment (p < .001). Overall differences in AFDM and chl a were observed between cobbles from different stream sites. Phosphorus enrichment stimulated benthic chl a biomass, but enrichment effects were greater when streams were enriched with N+P (p < .001). Chlorophyll‐a increased (4.4–57.9 mg m−2) with increasing P concentrations (p < .001) after P enrichment but was threefold greater after N+P enrichment, increasing from 13.3 to 171.1 mg m−2 across the enrichment gradient. Results support the need to consider both N and P limitation in freshwater systems and demonstrate that potential increases in nutrient concentrations may influence accumulation of algae on cobble substrates from the Buffalo River watershed.

Ozark Bass (Ambloplites constellatus) is an understudied, endemic fish species in the Upper White River Basin of northern Arkansas. This study was part of an effort by fisheries managers to gather baseline data about the Ozark Bass to aid in understanding population dynamics and contribute to the limited data available for use in determining the efficacy of harvest regulations. Select population characteristics of Ozark Bass in two northern Arkansas streams were determined, population characteristics of Ozark Bass were compared to Shadow Bass (Ambloplites ariommus) and Rock Bass (Ambloplites rupestris) data collected from previous studies in southern Missouri, and relative condition, length-at-age, and annual survival of Ozark Bass were compared between sample streams. Sampling occurred in Crooked Creek and the Buffalo River during summer 2013 via boat electroshocking. Length and weight data were recorded for all Ozark Bass collected, and fish ages were determined through selective otolith retrieval and age-length keys. Ozark Bass in Crooked Creek had greater relative condition than Ozark Bass in Buffalo River (P < 0.001). Neither Ozark bass lengths nor log-transformed weights differed (P > 0.05) between sexes for fish collected from only the Buffalo River. Ozark Bass mean annual survival was similar between Crooked Creek (55% ± 5% as 95% confidence interval (CI)) and the Buffalo River (50% ± 7% CI) for fish age 2 to 9. Calculated Ozark Bass lengths-at-age for fish from both streams were comparable to the Von Bertalanffy growth estimates, except the Buffalo River age 7 category where there was only one observation. The relationship between Ozark Bass age and length differed between sam-How to cite this paper: