Rising of antibiotic resistance is threatening the global health care system and increasing the worldwide death rate. Therefore, research and discovery of alternative antimicrobial agents from plant sources are encouraged. The Artabotrys suaveolens (Blume) belongs to the Annonaceae family and mainly distributed in tropical and subtropical regions of the world. It was indigenously used to treat postnatal weakness and cholera infection.The study aims to provide evidence of the plant as an alternative source of antibacterial agent based for its folkloric use to treat infection. This study was undertaken to fractionate the chloroform extract of the stem of Artabotrys suaveolens (Blume) and to investigate the in vitro antimicrobial activities of different solvent fractions against three ATCC and MDR bacteria.Liquid-liquid fractionation was performed resulting with petroleum ether, chloroform and water fraction of the stem of Artabotrys suaveolens (Blume). Qualitative phytochemical analysis was conducted for alkaloid, cardiac glycoside, flavonoid, saponin, sterol and tannin. Antibacterial activity was ascertained by disc diffusion assay, minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against three ATCC strains (MSSA ATCC 29213, K. pneumoniae ATCC 13883 and E. coli ATCC 35218) and three clinical isolated strains (MRSA, K. pneumoniae, A. baumannii). GraphPad Prism 8 was used for data analyses. Differences are statistically significant when p<0.05.Qualitative phytochemical analysis revealed that both petroleum ether and chloroform fraction contained alkaloid, sterol and tannin, while water fraction contained cardiac glycoside, saponin, and tannin. Petroleum ether fraction showed notable antibacterial activity against MSSA ATCC 29213 (inhibition zones=10.00±1 mm; MIC=0.5 mg/mL; MBC>2 mg/mL) compared to
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