BACKGROUND Recovery of optimum number of good quality of spermatozoa is an important component of In Vitro Fertilisation (IVF). This is achieved by sperm preparation methods involving separation and recovery of capacitated sperms. Double Wash Swim-up (DWSU) and Double Density Gradient Swim-up (DDGSU) are two most accepted methods. Cochrane systematic review (2007) finds no clear benefit of one method over the other in Intrauterine Insemination (IUI). Systematic review on effectiveness of these preparations in IVF is lacking. Effectiveness is generally assessed in terms recovery rates of the sperms. Capability of successful fertilisation of good quality oocytes should ideally be the functional endpoint for evaluating effectiveness of sperm preparation methods. The aim of the study is to-1. Compare the successful fertilisation rates of oocytes inseminated by semen preparation of Double Wash Swim-up (DWSU) visa -vis by Double Density Gradient Swim-up (DDGSU) method. 2. Evaluate the effectiveness of fertilisation of oocytes by Double Wash Swim-up method (DWSU) visa -vis Double Density Gradient Swim-up (DDGSU) method.