In this present study the effects of pH, temperature, single and dual metal ion concentrations, dose of Planococcus biomass and desorption agents on the uptake of Cd(II) and Ni(II) was measured using atomic absorption spectrophotometry. To show surface adsorption of bacteria, SEM-EDX and FTIR analysis were done before and after metals adsorption. The maximum biosorption capacity of Planococcus sp determined as 0.67, 0.48 mmol g −1 for cadmium(II) and as 0.58, 0.47 mmol g −1 for nickel(II) for single and binary ion situation. The correlation coefficient for the second-order kinetic model was 0.993. The Langmuir and Freundlich isotherm models were also applied to the equilibrium of system and data were better fitted with the Langmuir isotherm.