The goal of the present study was to ascertain the effect of the addition of certain natural materials such as sugar or Jaggery Syrup, Black Gram, or extracts there of as fortified materials on the consistency of apple vinegar and the types of microorganisms in it. The findings revealed that the addition of whole legume or their extracts during processing enhanced the properties of the resulting vinegar . The findings showed that the better pH was for apple vinegar produced with the inclusion of sugar and protein extract, which had a pH of 3.1. This was accompanied by vinegar resulting from the addition of jaggery with whole legume, where the pH was 3.8, followed by vinegar resulting from the addition of whole legume plus sugar, where the pH was 3.9. Though apple vinegar obtained by adding protein extract with jaggery had a pH of 4.As it was influenced by the acidity of the resultant vinegar due to the content of legumes and sugary compounds, the maximum acidity was 6.5 in the vinegar to which entire legume jaggy was applied. Then vinegar, to which whole legume sugar was added, and vinegar, to which jaggy with protein extract was added, the acidity reached 3. Finally, vinegar made by combining sugar with protein extract, where the acidity reached 2. The best types of vinegar were selected from among the samples (vinegar developed by the addition of protein extract jaggary) and the contents of its microbiology were analysed. The results showed that the vinegar was free of bacteria and yeasts, while its content of moulds was . The four samples of vinegar also showed good antioxidant activity. It was 86.77%, 85.38%, 79.0% and 19.11% in vinegar containing jaggery with extract protein, sugar with extract protein, jaggery with whole legume and sugar with whole legume respectively. The total soled of vinegar were different, 4.8%, 4.5%, 4.0% and 3.0% in vinegar containing jaggery with whole legume, jaggery with extract protein, sugar with whole legume and sugar with extract protein. The colour analysis of the vinegar sample containing the jaggery with extract protein was 75.91, 4.87, 51.4 for the lighter side, red colour and greener side, respectively.

Many of the aquatic pathogenic microflora belong to the non-lactose fermenting (NLF) Enterobacteriaceae and emergence as well as the spread of antibiotic resistance in them is now a serious threat to public health worldwide. Growth of textile industries has heavily flourished acting as hub for the ready-made garments factories in Bangladesh. Textile industry effluents may contain heavy metals and it is not clear whether their disposal is strictly monitored or not and their impact on the aquatic microflora have not been properly investigated and monitored. To assess the impact of heavy metals on the emergence and spread of antibiotic resistance in NLF Enterobacteriaceae, samples were collected from four different locations of drains running down effluents from five (n=5) different textile industries located in Savar, Dhaka, Bangladesh. Total bacterial counts (TBCs), minimal inhibitory concentrations (MICs) of heavy metals, antibiotic susceptibility, binary exposure and plasmid profile experiments were performed. TBCs from day 1 to day 5 revealed that most colony forming units (CFUs) uncountable at 10-6 dilution could be counted at 10-10 dilution. A total of 100 NLF bacterial isolates were categorized as type-1 and type-2 using 4 differential media. The MICs of 100% isolates in both type-1 and type-2 for nickel (Ni), lead (Pb) and chromium (Cr) were 0.6 mM, 0.6 mM and 1.0 mM, respectively. In case of type-1, most (38%) showed resistance to azithromycin (AZM) and least (2%) to cefotaxime (CTX). In case of type-2, most (48%) showed resistance to AZM and least (8%) to CTX. Binary exposure experiments revealed the combined effect of heavy metals and antibiotics. Where in most cases, zone of inhibition increased or remained unchanged. In few cases, zone of inhibition decreased. This finding indicated that the heavy metals typically exert negative or no effects on antibiotic resistance in the isolates tested. Plasmid profiling of the type-1 and type-2 NLF isolates resistant to both the antibiotics and heavy metals revealed that one of the five type-1 isolates contained very large plasmid (14 Kb) and four of the eight type-2 isolates contained 13 Kb plasmids and two contained 12 Kb plasmids. These findings indicated that these isolates possibly possess the ability to sequester metals before being discharged into the soil and/or water environment. Keywords: Antibiotics, effluents, heavy metals, minimum inhibitory concentration (MIC), resistance.