Nanoparticles are extensively used for various applications in science, engineering and medicine. Synthesis of nanoparticles with high purity is essential to utilize the same in different fields of science and technology. In the present study, liquid chromatography is utilized to purify the nanoparticles. Predominantly, gold nanoparticles were synthesized from gold auric cholide and preserved in phosphate or citrate buffer. A method to purify gold nanoparticles is essential because of the possible interference from gold auric chloride and other impurities in buffer. Herein, a method has been developed using high performance liquid chromatography to purify gold nanoparticles with 100 nm in size from gold auric chloride and residues. UV-Vis spectroscopy was also done to ascertain the purity of the nanoparticles.