Alternating current (AC) electrokinetics is a facile way of patterning colloidal particles into advanced structures. We demonstrate the combined use of AC dielectrophoresis (AC-DEP) and AC electrohydrodynamics (AC-EHD) in a microwell electrode geometry for size-tunable assembly of gold nanoparticles (AuNPs) into one-dimensional microwires and two-dimensional films. The AC-DEP force scales with both particle size and field frequency, whereas the AC-EHD force depends only on the field frequency. So, a critical particle diameter (d c ) exists, below which the EHD phenomenon becomes more important and beyond which the DEP force is dominating. We performed theoretical and experimental studies to determine "d c " and how it gets affected by operating parameters like field frequency, voltage, particle number, electrolyte concentration, electrode size, and geometry. Our results show that the morphologies of the colloidal structures transition from films to microwires as the NP diameters vary from nanometers (d c ), and no assembly takes place at intermediate sizes (∼d c ). While the film formation is governed purely by surface EHD flows, microwire synthesis is a result of EHD-assisted DEP phenomenon. Also, a minimum particle number, a low salt concentration, and an optimum frequency range is required to initiate assembly.

Design and development of a simple and ultrasensitive dot blot bioassay for endotoxin detection in serum.