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Fermentation technology is an important field comprising the use of microorganisms and enzymes to produce the compounds that have applications in the food, pharmaceutical, energy, and chemical industries. Although food fermentation processes have been used for generations as a prerequisite for sustainable food production, today it has become more demanding to obtain functional and therapeutic food products through the application of continuous creations and advancement of innovative fermentation processes. For these reasons, efforts are directed toward designing new processes, concepts, and technologies. Fermentation is a natural process whereby microorganisms such as lactic acid bacteria and yeast convert carbohydrates such as starch and sugar into alcohol or acids, both of these act as a natural preservatives. This process is still used today to produce foods such as wine, cheese, sauerkraut, yogurt, and other types of traditional foods. Traditional fermented foods are popularly consumed and form an integral part of our diet since early history. They are recognized as having multiple benefits related to nutritive values, therapeutic properties, and sensory attributes. In most fermented foods, the fermentation process is predominantly initiated by lactic acid bacteria. These organisms have been termed as probiotic bacteria—a group that appears to have specific health-promoting attributes.

Hyaluronic acid finds its complete application in areas such as therapeutics, cosmetics, and as a health supplement. In the present investigation, standardization for the production of hyaluronic acid by Streptococcus equisimilus MK156140 in complex media was performed. Some of the selected physicochemical parameters such as pH, temperature, speed, incubation time, sucrose, yeast extract, and beef extract were screened using Plackett-Burman foldover design. Further, the screened parameters interaction was investigated using central composite design (CCD) and closely compared with OVAT studies. At a pH of 7.38, with beef extract, 12.15%, and yeast extract 7.64%, the observed yield was 7.16 g/L, which was in close line with the predicted value of 7.21 g/L.