Background:Blighted ovum is one of the most common reasons for abortion during the first three months of pregnancy. Manganese superoxide dismutase (MnSOD) is an important antioxidant enzyme in the human immune system. The gene is located on 6q25 chromosome and acts on mitochondrial matrix. In the case of mutation or inactivity of this enzyme, mitochondrial and nuclear DNA will severely be destructed. The most common polymorphism of its gene is Val16Ala.Objective:The aim was to investigate a possible mutation in pregnant women who had abortion during the first trimester of pregnancy due to blighted ovum.Materials and Methods:In this case-control study, 34 women were entered as the case and control groups, respectively. Genome DNA was extracted from saliva samples and its genotype was determined using Tetra-primer amplification refractory mutation system polymerase chain reaction technique. Results:In the case group, 16 (48%) cases had Val/Val genotype, 17 (50%) were heterozygote and had Val/Ala genotype, and 1 (2%) had Ala/Ala genotype. Among controls, 7 (22%) items had Val/Val genotype, 6 (17%) had Val/Ala genotype, and 21 (61%) had Ala/Ala genotype. The frequency of TT, CT, and CC genotypes was 48%, 50%, and 2% in case group and 22%, 17%, and 61% in control group, respectively. Statistical analysis revealed a significant relationship between Val16Ala polymorphism of MnSOD gene and blighted ovum (p= 0.0003).Conclusion: It has concluded that a significant relationship exists between Val16Ala polymorphism of MnSOD gene and blighted ovum.

Objective: To evaluate salivary GPx-1 gene polymorphism in pregnant women suffering from blighted ovum. Method: In this case-control study, 34 blighted ovum patients and 34 healthy controls were studied. Genomic DNA was extracted from the saliva. The genotypes were determined by restriction fragment length polymorphism (RFLP-PCR) technique. Mad Calc (version 12.1) was used for statistical analysis. Result: The frequency of CC, CT, and TT genotypes of GPx-1 gene were 41%, 44% and 14%, respectively in blighted ovum patients and in healthy volunteers were 44%, 47%, and 8.82-9%, respectively. After statistical analysis, the study showed no significant association between this polymorphism and blighted ovum (with p = 0.63). Conclusion: These results indicated no significant association between GPx-1 (Pro198Leu) polymorphism and blighted ovum. However, further research is required to clarify the role of gene polymorphism in blighted ovum. [Indones J Obstet Gynecol 2016; 1: 15-18] Keywords: abortion, blighted ovum, glutathione peroxidase-1, GPx- 1, RFLP-PCR