Our current study deals with the green synthesis of copper nanoparticles using extract of Labeo rohita fish scales. There is no need to add any external reducing and stabilizing agents as the gelatin found in fish scale extract possesses plenty of reducing and stabilizing properties. The synthesized copper nanoparticles (Cu-NPs) were characterized by Zeita sizer and SEM. Cu-NPs were applied to decolorize Reactive Blue 19 dye. Different experimental conditions like concentration of reactive Blue 19 dye, concentration of copper nanoparticles, pH and temperature were optimized. The degrading potential of copper nanoparticles increased their applicability for the decolorization of Reactive Blue 19 dye. Reactive Blue 19 dye was maximum decolorized (90.18%) at aconc. of 0.03%, 4 mg Cu-NPs, pH 10 at 50ºC. The effectiveness of the method was evaluated by water quality assurance parameters such as total organic carbon (TOC) and chemical oxygen demand (COD). The reaction products were characterized by FTIR spectral studies.

White rot fungi have exclusive capacity to decolorize synthetic azo dyes. Five different fungal strains namely S. commune IBL-01 (SC), P. ostreatus IBL-02 (PO) P. chrysosporium IBL-03 (PC), T. versicolor IBL-04 (TV) and G. lucidum IBL-05 (GL) were used for the decolorization of synthetic dyes effluent. P. ostreatus IBL-02 showed maximum decolorization of synthetic textile effluent, so it was selected for the optimization of experimental factors. Different physico-chemical parameters were optimized using Pleutorus ostreatus IBL-02 (PO), for the maximum de-colorization of synthetic dyes effluent. Under optimum conditions, P. ostreatusIBL-02 (PO) decolorized the synthetic dyes effluent by 92.7%. Effect of various amendments like carbon and nitrogen sources on the decolorization and mineralization of synthetic dyes effluent by Pleutorus ostreatus IBL-02 was also studied. Ligninolytic enzymes at the end of each experiment were studied to find their role in decolorization and mineralization of synthetic dyes effluent. UV-Visible spectral analysis was used to indicate the decolourization of treated dyes effluent sample. A diminution in spectral line height displaying absorption maxima designated decolorization (%) while a move of spectral line to the UV-region depicted the degradation of synthetic dyes effluent.