Objectives:-This study was conducted to evaluate the clinical and laboratory effect of three different treatment modalities in management of dentin hypersensitivity. Materials and methods: one hundred-twenty samples were used in the study procedures, for dentin permeability test and Scanning Electron Microscopic examination (SEM). The samples were randomly divided into two groups Group A 1 , totally removed smear layer Group A 2 , partially removed smear layer. Each group was further subdivided into four subgroups according to the treatment agent applied: ammonium hexafluorosilicate (SiF) gel, propolis extract, Curodont D'senz and the control group that were left untreated. One hundred and five (105) hypersensitive teeth were enrolled for this study. The subjective assessment of dentin hypersensitivity was done before application of the agent (baseline) then immediate, 1 week, 2 weeks, 4 weeks and 3 months respectively. Patients were asked to rate their perception to tactile, air and cold stimuli by using Verbal Rating Scale (VRS) and Visual Analogue Scale (VAS). Results: Permeability results revealed that there were high statistical significant difference between the study groups with lowest statistically significant mean depth of dye penetration values were found with propolis treated samples. The results of the clinical study revealed that there was high significant difference between pain scores before and after treatment for all tested groups. Conclusion: Propolis extract, Curodont D'senz and SiF gel were effective in reducing dentin permeability, occluding dentinal tubules and alleviating the hypersensitivity symptoms, with propolis extract paste being the most effective within 1 to 2 weeks and sustained up to 3 months.