The purpose of this study was to examine the potential of tamarind seeds as a prebiotic by conducting tamarind seeds with a temperature and incubation time. Prebiotic testing was carried out using Lactobacillus casei grown on media with the addition of tamarind seeds. The research used a completely randomized design. The temperature was divided into two levels (60 and 70°C) and the incubation time is divided into three levels (120, 180, and 240 minutes). The treatments were T1 (control), T2 (60°C, 120 minutes), T3 (60°C, 180 minutes), T4 (60°C, 240 minutes), T5 (70°C 120 minutes), T6 (70°C, 180 minutes), and T7 (70°C, 240 minutes). Each experiment was repeated twice. Data were analyzed by analysis of variance, if there was a significant effect then continued with Duncan test. Based on the results of this study: tamarind seeds which were incubated at a temperature of 60°C for 240 minutes increased the number of colonies significantly (P<0.05) compared to the incubation time below, incubation tamarind seeds at 70°C increased the number of bacterial colonies significantly (P<0.05) in all-time ranges, however the optimal time 180 minutes. Tamarind seeds have potential as prebiotics, all the parameters showed the ability of tamarind seeds as a prebiotic.

Tujuan penelitian ini adalah untuk mengetahui pengaruh fermentasi ampas kelapa ( Cocos Nucifera L ) dengan Neurospora sitophila terhadap kandungan fraksi serat. Penelitain ini telah dilaksanakan dari bulan Juli – September 2021 di Laboratorium Fakultas Pertanian dan Peternakan Universitas Muhammadiyah Bengkulu dan analisa Van Soest telah dilakukan  di Laboratorium  Ternak Perah Peternakan Institut Pertanian Bogor ( IPB ).Percobaan dilakukan dengan menggunakan Rancangan Acak Lengkap ( RAL ) dengan 4 perlakuan dan 4 ulangan. Fermentasi Ampas Kelapa dibuat dengan formulasi 1,5 kg ampas kelapa dan 10% molases. dengan perlakuan dosis Neurospora sitophila P1 = ( 2% ), P2 = ( 4%), P3= ( 6%), P4 = (8%).Parameter yang diamati adalah Neutral Detergent Fiber (NDF), Acid Detergent Fiber (ADF), Hemiselulosa, Selulosa, Lignin.Hasil penelitian menunjukan bahwa perlakuan dosisNeurospora sitophilaberpengaruh nyata terhadap Kandungan NDF dan Hemiselulosa, Tetapi tidak berpengaruh nyata terhadap kandungan ADF, Selulosa dan Lignin Ampas kelapa fermentasi, Kandungan fraksi serat ampas kelapa fermentasi dalam penelitian ini adalah NDF  = ( 61,05 % - 70,89% ), Hemiselulosa = (21.68 % - 30,05 %), ADF = ( 37,57 %  –  40,84 % ), Selulosa = ( 30,87 % - 33,78 %)dan lignin = ( 5,14 % - 5,33% ). Kesimpulan  penelitian ini adalah Fermentasi Ampas Kelapadengan beberapa level Neurospora sitophila berpengaruh terhadap Kandungan Neutral Detergen Fiber (NDF) dan Kandungan Hemiselulosa tetapi tidak berpengaruh terhadap kandungan Acid Detergent Fiber (ADF), selulos, Lignin, Level Neurospora sitophila yang terbaik adalah P3 (6 % ).