Objective: Candida albicans tolerates unfavorable environmental conditions by forming biofilms, which can cause problems following endodontictreatments. C. albicans is the most common fungus found in failed root canals. Using natural irrigants with good antifungal properties for use assynthetic irrigant alternatives during root canal cleaning and shaping is very important. This study aimed to analyze the effects of Aloe vera onC. albicans biofilm.Methods: C. albicans biofilm was divided into the following five groups: Group I, no treatment (control group); Groups II, III, and IV, A. vera with aconcentration of 100%, 75%, and 50%, respectively; and Group V, Ethylenediaminetetraacetic acid (EDTA) 17%.Results: The average biofilm colony count for C. albicans for the A. vera 100%, 75%, and 50% groups was higher than that for the EDTA 17% groupbut lower than that for the control group.Conclusion: A. vera has antifungal effects on C. albicans biofilm, with the greatest effect occurring at 75% concentration.