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BACKGROUNDHereditary spherocytosis is an inherited disorder of red blood cells which results in cytoskeleton protein deficiency. We wanted to assess the role of reticulocyte parameters in the diagnosis of hereditary spherocytosis. METHODSCases in which osmotic fragility test was increased and peripheral smear showed spherocytes and polychromasia which were suggestive of hereditary spherocytosis were selected from Laboratory Information System (LIS). The study period was from January 2012 to December 2016. RESULTSThe present study showed increase in retic % and Red Cell Distribution Width (RDW) in cases of hereditary spherocytosis when compared to the normal controls and there was a significant statistical difference (p < 0.001), between the two groups. Area Under the Curve (AUC) was calculated for MCHC, IRF, MRV and Retic#/IRF by using ROC curve analysis and was maximum for Retic#/IRF (0.931), followed by MCHC (0.902), IRF (0.867) and MRV (0.865). Cut off values derived for RET#/IRF, IRF, MRV and MCHC were ³0.28, ³0.33, £ 98.8 and ³33.5 respectively. Highest sensitivity and specificity was obtained for Ret#/IRF which was 96% and 88% respectively. Percentage of cases with MRV < MCV is 63.6%. CONCLUSIONSThe present study shows that reticulocyte parameters are useful in the diagnosis of Hereditary Spherocytosis. It proves that in addition to the presence of spherocytes in peripheral smear and an increased osmotic fragility test, reticulocyte parameters like RET#/IRF ³ 0.28, IRF³ 0.33 and MRV£ 98.8 are highly indicative of Hereditary Spherocytosis.

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