Patients with Behçet's disease (BD) suffer from episodic inflammation often affecting the orogenital mucosa, skin, and eyes. To discover new BD-susceptibility loci, we performed a genome-wide association study (GWAS) of 779,465 SNPs with imputed genotypes in 1,209 Turkish BD patients and 1,278 controls. We identified novel associations at CCR1, STAT4, and KLRC4. Additionally, two SNPs in ERAP1, encoding ERAP1 p.Asp575Asn and p.Arg725Gln, recessively conferred disease risk. These findings replicated in 1,468 independent Turkish and/or 1,352 Japanese samples (combined meta-analysis p < 2 × 10−9). We also found evidence for interaction between HLA-B*51 and ERAP1 (p = 9 × 10−4). The CCR1 and STAT4 variants were associated with gene expression differences. Three risk loci shared with ankylosing spondylitis and psoriasis (MHC-I, ERAP1, and IL23R, and the MHC-I-ERAP1 interaction), as well as two loci shared with inflammatory bowel disease (IL23R and IL10) implicate shared pathogenic pathways in the spondyloarthritides and BD.

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The inflammatory responsiveness of the cells to Lipopolysaccharides (LPS) is commonly used for in vitro experiments. However, the correct dose of the LPS for cell line experiments is elusive. The LPS concentration that gives the maximal response is a critical point of in vitro inflammatory experiments. The aim of this study was to reevaluate the concentration dependent effect of LPS on the 293T human renal cell line. Methods:We evaluated the cell-detachment assay of LPS-stimulated 293T cell line monitored by xCELLigence Real-Time Cell Analyzer (RTCA) system. We applied increasing concentration of LPS followed by Roche xCELLigence Instrument based on the Real-Time Cell Analysis System. Results:Our results demonstrated that the 2, 4 and 8µg of LPS inhibit cell division which diverts cells to a steady-state phase, 1 µg acts as mitogen. Lower concentrations are no effect on cells. Conclusion:These work showed that LPS concentrations had various effects on cell proliferation and need to be estimated for each experiment before carry out the experiments.