Introduction: N-Acetylcysteine (NAC) has been used to treat paracetamol overdose for four decades. But the administration of NAC regimens is complicated. Therefore, medication errors are an ongoing issue in the acute-care setting and directly impact the safety of patients. Objective: To evaluate the rate of NACrelated medication errors during the management of paracetamol overdose at a teaching hospital in Malaysia. Methods: This clinical audit study was performed retrospectively between June 2008 and June 2011. Patients who presented to the adult emergency department with paracetamol overdose and had received at least the loading dose of intravenous NAC were eligible. Children below the age of 12 years old were not included. Also, patients displaying chronic excessive paracetamol usage or recurrent paracetamol overdose were excluded. We determined the type and frequency of medication errors during intravenous NAC administration. Results: The study population ranged from 13 to 87 years old (mean age: 25.06; median: 23) and was predominantly female. The overall prevalence of intravenous NAC-related medication errors was found to be 84.3%. Moreover, the following frequencies were observed based on error type: 5.9% wrong dose, 37.3% incorrect infusion rate, 8.5% interruption during treatment, and 66.5% unnecessary administration. Conclusion: We observe a surprisingly high prevalence of medication errors related to the administration of intravenous NAC. Thus, this emerging issue needs be addressed in order to ensure patient safety as well as to prevent unnecessary hospital admissions and/or burdening treatment costs. (Hong Kong

The Caenorhabditis elegans Gene Knockout (KO) Consortium is tasked with obtaining null mutations in each of the more than 20,000 open reading frames (ORFs) of this organism. To date, approximately15,000 ORFs have associated putative null alleles. A directed approach using CRISPR/Cas9 methodology is the most promising technique to complete the task. While there has been substantial success in using CRISPR/Cas9 in C.elegans, there has been little emphasis on optimizing the method for generating large insertions/deletions in this organism. To enhance the efficiency of using CRISPR/Cas9 to generate gene knockouts in C. elegans we have developed an online species-specific guide RNA selection tool (http://genome.sfu.ca/crispr). When coupled with previously developed selection vectors, optimization for homology arm length, and the use of purified Cas9 protein, we demonstrate a robust, efficient and effective protocol for generating deletions. Debate and speculation in the larger scientific community about offtarget effects due to non-specific Cas9 cutting has prompted us to investigate through whole genome sequencing the occurrence of single nucleotide variants and indels accompanying targeted deletions. We did not detect any off-site variants above the natural spontaneous mutation rate and therefore conclude this modified protocol does not generate off-target events to any significant degree in C. elegans.4 Introduction:

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A clear-eyed look at how AI can complement (rather than eliminate) human jobs, with real-world examples from companies that range from Netflix to Walmart. Descriptions of AI's possible effects on businesses and their employees cycle between utopian hype and alarmist doomsaying. This book from MIT Sloan Management Review avoids both these extremes, providing instead a clear-eyed look at how AI can complement (rather than eliminate) human jobs, with real-world examples from companies that range from Netflix to Walmart. The contributors show that organizations can create business value with AI by cooperating with it rather than relinquishing control to it. The smartest companies know that they don't need AI that mimics humans because they already have access to resources with human capability—actual humans. The book acknowledges the prominent role of such leading technology companies as Facebook, Apple, Amazon, Netflix, and Google in applying AI to their businesses, but it goes beyond the FAANG cohort to look at AI applications in many nontechnology companies, including DHL and Fidelity. The chapters address such topics as retraining workers (who may be more ready for change than their companies are); the importance of motivated and knowledgeable leaders; the danger that AI will entrench less-than-ideal legacy processes; ways that AI could promote gender equality and diversity; AI and the global loneliness epidemic; and the benefits of robot–human collaboration. Contributors Cynthia M. Beath, Megan Beck, Joe Biron, Erik Brynjolfsson, Jacques Bughin, Rumman Chowdhury, Paul R. Daugherty, Thomas H. Davenport, Chris DeBrusk, Berkeley J. Dietvorst, Janet Foutty, James R. Freeland, R. Edward Freeman, Julian Friedland, Lynda Gratton, Francis Hintermann, Vivek Katyal, David Kiron, Frieda Klotz, Jonathan Lang, Barry Libert, Paul Michelman, Daniel Rock, Sam Ransbotham, Jeanne W. Ross, Eva Sage-Gavin, Chad Syverson, Monideepa Tarafdar, Gregory Unruh, Madhu Vazirani, H. James Wilson

TWELVE West African dwarf (Fouta Djallon) kids, 4 to 6 months of age and weighing between 5.96 and 7.24kg, were used in the study of three dietary treatments involving a basal diet of browse plant (gliricidia Septum) supplemented with concentrate feed at 25 and 50% levels of dry matter intake to appetite. The diets were fed for a period of three months in order to estimate nutrient intake and utilization, protein and energy requirements including growth performances of the kids.  Kids maintained on gliricidia sepium alone (G1000CO) needed significantly (P/0.05) more dry matter (DM) consumption to appetite (294.66 ± 14.941 g/day) than kids maintained on either 75% gliricidia sepium plus 25% concentrate (G75 C25) or 50% gliricidia sepium plus 50% concentrate (GC)50 (236.26±0.385kg) than either kids on treatments G100C0 or (GC)50 (6.84±0.214 and 6.92±0.313kg respectively). DM intake as a percentage of liveweight was about 3.60 +0.237%.  The kids were in positive nitrogen balance and the nitrogen retention value ranged from 31.36 ±1.633 to 50.22 ±0.994%. Metabolic faecal nitrogen (MFN) and endogenous urinary nitrogen (ENU) were 0.232±0.005g/100g DM intake and 0.070 g/day wkg734 respectively. The biological value (BV) of the diets ranged from 56.79 to 75.62%. The digestible crude protein (DCP) requirement for maintenance was 0.853±0.133 g/day/wkg734 while the DCP requirement for liveweight gain was 0.030±0.001 g/day/wkg734 per g of liveweight gain. The digestible energy (DE) and metabolizable (ME) energy requirements for maintenance were 124.42±3.274 kcal/day/wkg734 and 100.78 + 4.588 kcal/day/wkg734 respectively.  Results showed that the utilization of the browse plant (gliricidia sepium) by the kids was best when fed at 75% browse plus 25% concentrate.