The data were collected from a brown mustard seeds collection of 18 accessions during two years and in three distinct sites of production in France. The 18 accessions of mustard seeds were selected to be representative of genetic, agronomical and technological variabilities. All accessions were produced in the “ Bourgogne ” area. This article describes agronomical data (PMG, yield), genotyping data, global composition of mustard seeds (lipids, proteins and polysaccharides) and fine composition of the previous macronutrients potentially involved in the technological properties (fatty acids, storage proteins and osidic composition of polysaccharides). Additional data regarding the potential rheological property of each accessions were also reported. These data can be reused by food industries, breeders and geneticists in order to understand pedoclimatic effects (year and location) and the relation between mustard seed composition and the end-uses properties (paste mustard quality).

In this study, extraction of soluble proteins from rapeseed cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly, response surface methodology (RSM) using a central composite design (CCD) approach was applied to investigate the influence of process variables on ultrasound-assisted extraction (UAE). Statistical analysis revealed that the optimized conditions providing a protein yield of 4.24 g/100 g DM were an ultrasound power of 5.6 W·cm−2 and temperature of 45 °C. Quantitatively UAE followed by two stages of conventional extraction gave the best total protein yield of 9.81 g/100 g DM. Qualitatively, the protein efficiency ratio (PER) used as measure of the nutritive value (12S/2S ratio) which indicates protein quality in terms of S-containing essential amino acids, was similar to that of the conventional extraction method. Small amounts of protein aggregate were observed in the HPLC profile of the extract.

In order to provide varieties of Brassica juncea that meet agronomic and technological qualities, it is necessary to define criteria that are predictive of the quality of the finished product. The objective of our study is to characterize the macromolecular components of the mustard seed in order to identify the compositional profile responsible for the technological quality of mustard pastes. A collection of cultivars that maximizes genetic and technological diversity were grown on three plots and over two years. Our results showed (i) the impact of the genetic and pedoclimatic factors on seed macromolecular components and on rheological properties estimated by measuring the flow in a Bostwick consistometer, and (ii) the potential role of seed storage proteins and cell wall polysaccharides in the mustard paste consistency. Data analyses allow us to propose new criteria for evaluating the technological quality of seeds and to identify interesting genotypes as candidates for future breeding programs.