The study contains the description of the ostrich fat by estimating the chemical content, which included moisture, protein, fat, ash, cholesterol, minerals and lipid-soluble vitamins, including vitamin (E andA) and the estimation of lipoproteins. Chemical properties were also studied which include peroxide value (PV), free fatty acids (FFA), saponification number (SN), iodine number and Physical properties include viscosity, density and specific gravity, refractive index, melting point and ester value (EV). Fatty acids were identified by using gas chromatography (GC). The results showed the percentage of moisture, protein, fat and ash were 5.56%, 5.16%, 87.88% and 1.03%, respectively. The mineral includes sodium, potassium, calcium, magnesium, phosphorus, iron and selenium reach to 8.5, 2.5, 40.11, 12.96, 78.6, 0.532 and 7.86 ppm respectively, cholesterol content was 50.63 mg / 100 g, vitamins content A and E was recorded 72.13 and 91.30 mg / ml respectively. The fat has good chemical and physical properties, such as viscosity, density and specific gravity, refractive index, melting point, saponification number, iodine number and ester value. While the results of lipoproteins in the ostrich fat HDL was 65 mg / dl, LDL 52 mg / dl and VLDL 35 mg / dl and triglycerides reach to 75 mg / dl. The peroxide number was 0.86 equivalent Meq O2 / Kg, and the percentage of the free fat acids reach to 0.38%. The fatty acid compassion was analyzed by gas chromatography (GC) and also showed nine saturated and unsaturated fatty acids including oleic acid, palmitic acid, arachidonic acid, erucic acid, heneicosanoic acid, undecanoic acid, lauric acid, caproic acid and myristic acid.